A DNA microarray contains immobilized DNA that corresponds to different genes. The microarray is hybridized with fluorescently labeled DNA probes reverse transcribed from the control and experimental mRNA samples. The control probe is labeled with a red fluorochrome, while the experimental probe is labeled with a green fluorochrome. What will be the color of the spot representing a gene with moderately upregulated expression in the experimental sample relative to the control one

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Answer 1

The color of the spot of a gene with moderately upregulated expression will be lime-green. A DNA microarray is a technique used to determine the expression level of many genes simultaneously.

A DNA microarray is a laboratory technique used to identify the genes that are being expressed in a cell.

A DNA microarray contains many tiny spots of specifically labeled cDNA samples referred to as probes, which can be hybridized by base complementarity to transcribed (mRNA) gene sequences.

In the DNA microarray, the probes can be fluorescently marked gene sequences.

After hybridization, the DNA microarray is then scanned in order to determine the level of gene expression of each mRNA that hybridizes with their corresponding probes on the slide.

In this case, green spots evidence genes that are being strongly expressed in the sample.

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Related Questions

What are 4 characteristics of a non renewable source

Answers

Answer:

There are four major types of nonrenewable resources: oil, natural gas, coal, and nuclear energy.

Explanation:

When salt water i added to a plant cell why does the cell membrane srink

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If a higher concentration of salt is placed outside of the cell membrane, the water will leave the cell to bond with it. The loss of water from this movement causes plant cells to shrink and wilt.

Genetic variation is needed for the long-term survival of a species.

True
False

Answers

True without genetic variation a single disease could dramatically reduce a species if they are susceptible to that disease. Genetic variations allows for a diverse gene pool that leads to long term survival for a species

PLEASE HELP ASAP (30 POINTS)

A sound with more vibrations per second sounds higher than a sound with fewer vibrations per second.
Question 8 options:
True
False

Answers

Answer:

true

Explanation:

One possible result of chromosomal breakage is for a fragment to join a nonhomologous chromosome. What is this alteration called

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Answer:

A translocation

Explanation:

Anwser this Please thank you

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Answer:

I think the first one is True?

CAN SOMEONE PLEASESS HELP ME!! Thank uu!!!

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The first one is D and the ones with lowering can help you with the second one either one should work

by moving substances into and out of cells,_____, the process of keeping stable conditions inside a cell, is maintained

Answers

Answer:

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Answer:

Homeostasis

Explanation:

This process maintains sugar, salt nutrients and other substances within a certain range to maintain safety

Which of the following types of agriculture is most likely to use economies of scale?

Biodynamic
Conservation
Industrial
Slash-and-burn

Answers

Among the options listed, the most likely to use economies of scale (given our understanding of this concept) is Industrial Agriculture.

The concept of economies of scale is the idea that a business entity uses mass production of a product in order to be able to reduce the cost of every individual unit. This is a very common practice in industrial work environments.

Like most industrial work systems, Industrial Agriculture is focused on efficiency. The companies in question often use mass production as a method of reducing costs, given that by completing the process on a bigger scale, they can create more units of a product while sharing the same cost.

This system is the exact definition of the use of an economy of scale, therefore we can confirm that the Industrial Agriculture option is correct for the question at hand.

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Agriculture is most likely to use economic scale Industrial.

The premise of economies of scale is that a corporate organization uses large production of a product to minimize the cost of each individual unit. This is a typical practice in industrial workplaces.

Industrial Agriculture, like most industrial work systems, is concerned with efficiency. The corporations in issue frequently use mass manufacturing to cut costs, because by completing the process on a larger scale, they can produce more units of a product at the same cost.

This system exemplifies the use of economies of scale, thus we can confirm that the Industrial Agriculture choice is correct for the subject at hand.

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The primary electron acceptor in photosystem II is __________. Group of answer choices P700 P700 P680 P680

Answers

Answer:

680

Explanation:

When the P680 special pair of photosystem II absorbs energy, it enters an excited (high-energy) state. Excited P680 is a good electron donor and can transfer its excited electron to the primary electron acceptor, pheophytin.

The nitrogenous base adenice is found in all the members of which group

Answers

Answer:

ATP, RNA, NAD and DNA.

Explanation:

A nitrogenous base; it is an organic molecule containing a nitrogen, carbon and other atoms that has the chemical properties of a base that is why they are called as bases. The nitrogenous bases are; i) adenine, ii) guanine, iii) thymine, iv) uracil and v)cytosine.

Adenine is a nitrogenous base which is found in ATP, RNA. NAD, FAD and DNA. adenine pairs with thymine in DNA synthesis.

Through which of the following room-temperature substances would sound travel the slowest? a water b rubbing alcohol c oxygen d glass

Answers

Answer:

oxygen I think soooooo yeah

Answer:

Oxygen

Explanation:

What process allows the cell to remain efficient

Answers

Answer:

cell growth control

Explanation:

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in response to action potentials arriving along the transverse tubules, the sarcoplasmic reticulum releases:

Answers

It release carbon dioxide

16. Which organelle may have allowed early eukaryotes to make food and produce oxygen? (a) the Golgi apparatus (b) the central vacuole (c) the plastids

Answers

Answer:

the plastids

Explanation:

Answer:  

The plastids are the organelles that compose the cells of plants and algae. These contain chloroplasts, which allow them to capture energy from sunlight. If they are ever removed, these cells will eventually lose their ability to photosynthesize food without help from another organism - they have lost an essential organelle. Without the presence of this organelle, eukaryotes may not have been able to use sunlight as a mechanism for producing oxygen or making nutrients such as glucose available for consumption.

The organelle that may have allowed early eukaryotes to make food and produce oxygen is the plastid. The correct option is c.

What is plastid?

The organelles that make up the cells of plants and algae are called plastids. These have chloroplasts, which enable them to absorb solar energy. These frequently include several kinds of pigments that can alter the color of the cell as well as pigments that are employed in photosynthesis.

These cells will eventually lose their capacity to photosynthesize food without the assistance of another creature if they are ever taken out since they have lost an important organelle.

Eukaryotes might not have been able to exploit sunlight to produce oxygen or make nutrients like glucose available for consumption without the presence of this organelle.

Therefore, the correct option is (c) the plastids.

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the primates that spend the most time walking upright are the _____.

Answers

Answer:

Hominids

Explanation:

can someone please help me!! ASAP!!
Thank uuu!!!

Answers

ANSWER

A

Explanation:

no explanation

Answer: D
Step-by-step explanation:

What are the missing coefficients for the skeleton equation below? N2+ H2-->

NH3

(A)3,1,2 (B)1,3,3 (C)1,3,2 (D)1,1,2 (E)2,6,6

Answers

I choose the option C.
N2 + 3H2 = 2NH3

What is the importance of microtubules in cell division?(1 point)

They provide a pathway for organelles to move into the newly formed cell.


They reform the nucleus in the divided cell.


They organize and pull the chromosomes apart.
.

They start the process of cytokinesis.

Answers

Microtubules are very important in a number of cellular processes. They are involved in maintaining the structure of the cell and, together with microfilaments and intermediate filaments, they form the cytoskeleton. They also make up the internal structure of cilia and flagella.

Answer:

C) They organize and pull the chromosomes apart.

Explanation:

in the comments!

Hope this helps for all the other students looking for the answer!

is there a nutritional difference in brown and white eggs

Answers

Answer:

there is no significant difference in nutrients between brown and white eggs.

Why is ammonification an important process?
a) Bacteria converts ammonia into nitrites and nitrates that plants can use
b) Fungi convert nitrogen in dead plants into ammonium, which converts proteins into ammonia
c) Nitrogen is converted into a usable form through lightning fixation
d) Plant enzymes reduce nitrogen compounds into amino acids

Answers

Answer:

c

Explanation:

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how will climate change affect the habitat distribution for the baltimore oriole?

Answers

Climate change has a great effect on the habitat distribution of Baltimore orioles bird.

Climate change will adversely affected the habitat distribution of Baltimore orioles because climate change causes heat waves, wildfires and intense downpours which is harmful for Baltimore orioles.

The climate changes will limit the populations of ranges of Baltimore oriole as well as changes the habitat. These Baltimore orioles moved to other places where the climate is favourable so we can conclude that climate change has a great effect on the habitat distribution of Baltimore orioles bird.

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Which of the following is the BEST description of osmosis?
A.) as active transport
B.) as requiring a transport protein
C.) as an example of facilitated diffusion
D.) as diffusion of water through a selectively permeable membrane

Answers

Answer:

D

Explanation:

Osmosis is the diffusion of water through a selectively permeable membrane.

ANYONE FROM K12 KNOW THE ANSWERS TO THIS PART OF THE 3.25 MITOSIS LAB??? I NEED HELP (no links)

Answers

Answer:

1. provide the correct order for the stages of mitosis

A psychologist working from the biological perspective would study all of the following to see how they affect behavior and cognitive processes except __________. A. Attention B. Brain cells C. Genes D. The nervous system Please select the best answer from the choices provided A B C D.

Answers

Answer:

Attention

Explanation:

Just took the test

A psychologist working from the biological perspective would study all of the following to see how they affect behavior and cognitive processes except Attention. The correct option is A.

Thus, When a psychologist approaches his or her job from a biological standpoint, he or she should not take into account attention as a factor because it is a psychological factor rather than a biological one.

Brain cells, genes, and the nervous system can all be thought of as biological elements.

It can be utilized to analyze how the brain, genes, and nervous system affect cognition and behaviour.

Thus, A psychologist working from the biological perspective would study all of the following to see how they affect behavior and cognitive processes except Attention. The correct option is A.

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why does the presence of telomeres protect the ends of linear chromosomes?

Answers

To prevent the loss of genes as chromosome ends wear down, the tips of eukaryotic chromosomes have specialized DNA “caps” called telomeres. Telomeres need to be protected from a cell's DNA repair systems because they have single-stranded overhangs, which "look like" damaged DNA. ...

A) What structures form the male part of the flower

B) What structures form the female part of a flower

C) how do male gametes find female gametes in a flower ?describe with de​

Answers

Answer:

a - The androecium

b -  the pistil

c - Male gametes is the pollen and it find the female gamete by wind, pollenators, and water.

Explanation:

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Height in humans is a polygenic trait with a distribution similar to the graph shown Which is true concerning the distribution of
height in human populations? (1 point)
O Most individuals will be below average height
O Most individuals will be at the extremes of height
O Most individuals will be above average height
O Most individuals will be of average height

Answers

Answer:

The answer is D

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Explanation:

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how are unicellular and multicellular organisms the same

Answers

Answer:

Unicellular organisms are made up of only one cell that carries out all of the functions needed by the organism, while multicellular organisms use many different cells to function. Unicellular organisms include bacteria, protists, and yeast.

Explanation:

How do CAM plants photosynthesize under bright hot conditions without drying out?

Answers

Answer:

by sealing their leaves against water loss and trapping carbon as organic acids

Explanation:

hope this helps :)

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