DNA sequencing by the chain-termination method utilizes DNA polymerase I to make a complementary copy of the target or template DNA molecule. A reaction with a 20 bp template and dideoxyadenosine nucleotides as terminators results in the production of a 5 bp fragment. Based on this result, we can conclude that the template contains A) a cytosine at position 5. B) a thymine at position 5. C) a cytosine at position 16. D) a thymine at position 16

Answers

Answer 1

Answer:

D) a thymine at position 16

Explanation:

The chain termination method (Sanger sequencing), is a sequencing technique used to determine the nucleotide sequence of a specific fragment of DNA. The classical method has three steps: 1- the target DNA fragment to be sequenced is used as a template for chain-termination PCR technique where modified deoxyribonucleotides (ddNTPs) are added to the PCR reactions to be incorporated by the DNA polymerase during in vitro DNA replication, 2-the DNA fragments of different sizes are separated by electrophoresis, and 3-determination of the DNA sequence by observing the identity of the terminal ddNTP in each band of the electrophoresis gel. For example, if the bottom band in the electrophoresis gel colocalizes in the column with ddATP as terminator, then this fragment finishes with ddATP, which means that the last nucleotide from the 5' end is Adenine.


Related Questions

Question 5

1 Q) Which of the following best describes an ecosystem?

1) A list of all plant and animal species found in the California desert

2) All of the white-tailed deer in a forest

3) Sunlight, temperature, rainfall, water sources in an area

4) An entire rainforest, including all biotic and abiotic factors


2 Q) Which of the following happened after wolves were removed from Yellowstone's ecosystem?

1) Biodiversity dramatically increased

2) The elk overgrazed the habitat vegetation of other species

3) Biodiversity was unaffected

4) The elk population rapidly decreased


3 Q) Which features are used to describe a biome?

1) Climate, wildlife, and vegetation

2) Longitude and latitude

3) Common name, scientific name, and conservation status

4) Bacteria, Archaea, and Eukarya


4 Q) Which statement is correct about biomes?

1) They represent large geographic areas

2) They all have about the same species richness

3) Water biomes are not as complex as land biomes

4) Biomes can be as small as a pond


5 Q) Which of the following is the largest biome on earth by area?

1) Tundra

2) Marine

3) Temperate Grassland

4) Desert

Answers

Answer:

1 Q) Which of the following best describes an ecosystem?

4) An entire rainforest, including all biotic and abiotic factors

2 Q) Which of the following happened after wolves were removed from Yellowstone's ecosystem?

2) The elk overgrazed the habitat vegetation of other species

.3 Q) Which features are used to describe a biome?

1) Climate, wildlife, and vegetation4

Q) Which statement is correct about biomes?

1) They represent large geographic areas

5 Q) Which of the following is the largest biome on earth by area?

)3 Temperate Grassland

Why is PTC important to be passed on in animals and humans?

Answers

Answer:

PTC (phenylthiocarbamide) is important to be passed on in animals and humans because it is a gene that determines the ability to taste certain bitter compounds. This gene helps to determine which foods are safe to eat, as some bitter compounds are toxic and can be dangerous if consumed.

Explanation:

what factors must be kept constant in the experiment to obtain valid results

Answers

i think the answer is constants

Interpret the Punnett square for Cross #2 to determine the predicted ratio of black, chocolate, and yellow puppies in the F1 generation.

People have been involved with the raising and breeding of domesticated plants…

Answers

According to the information, the ratio of black, chocolate, and yellow puppies in the F1 generation is found to be 3: 1: 4.

What is Punnett square?

The Punnett square may be defined as a type of table through which all of the possible outcomes for a genetic cross between two individuals with known genotypes are given.

When a yellow female with genotype Bbee is crossed with a black male with genotype BbEe. It produces offspring with the genotypes BBEe, BbEe, BBee, Bbee, BbEe, bbEe, Bbee, and bbee in the F1 generation. But the phenotype of yellow is determined by the presence of homozygous ee allele. While black color B is dominant over the chocolate color, b.

Therefore, according to the information, the ratio of black, chocolate, and yellow puppies in the F1 generation is found to be 3: 1: 4.

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Bood volume and blood pressure can be stimuli for the release of vasopressin, changes in osmolarity serve as the strongest stimulus.

a. True
b. False

Answers

Answer:

a. True

Explanation:

Vasopressin is a peptide hormone, synthesized in the hypothalamus, that has a strong effect on the kidney, cardiovascular homeostasis, and on the body generally, especially responding to changes in osmolarity of the blood.

Just like every other hormone, vasopressin is a hormone released on-demand, based on the state of the body, the need, its capability to solve a problem. It has its target organ and function. Changes in osmolarity cause kidneys to reabsorb water into the blood is the strongest stimulus responsible for the release of vasopressin. Vasopressin is also stimulated in response to blood volume and blood pressure.

                 

Ravi is driving her car, hits a patch of ice, and momentarily loses control of the vehicle. Despite spinning in a full circle, Ravi manages to
avoid hitting any objects and drives away unharmed. Ravi experiences a surge of adrenaline and a racing heartbeat to due to the activation
of which of the following?
O sympathetic nervous system
O parasympathetic nervous system
O thyroid gland
O somatic nervous system
O pancreas

Answers

Ravi is driving her car, hits a patch of ice, and momentarily loses control of the vehicle. Ravi experiences a surge of adrenaline and a racing heartbeat to due to the activation due to Symphthetic nervous system.

What is Symphathetic nervous system?

Autonomic nervous system includes the sympathetic nervous system. It might be referred to as your "automatic" nervous system because it controls a variety of processes that don't require conscious thought.

The ability of your sympathetic nervous system to react to risky or stressful conditions is its most well-known function.

When this happens, your sympathetic nervous system kicks in to help you escape danger by increasing your heart rate, pumping more blood to areas of your body that need it, and other responses.

Therefore, Ravi is driving her car, hits a patch of ice, and momentarily loses control of the vehicle. Ravi experiences a surge of adrenaline and a racing heartbeat to due to the activation due to Symphathetic nervous system.

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Next week produces a heat wave in Prince George's County. Most scientists would say:

A. Global warming is the cause

B. Human activity is the cause

C. Local temperatures over one week tell us more about weather than climate

D. Our climate is getting warmer

Answers

the answer is A, global warming is the cause

Use the scenario to answer the question.

A student decides to test the effects of watering on plants. She chooses 6 tomato plants and places all of them in the same part of a garden with plenty of space between them. They get the same amount of sunlight and the same amount of fertilizer each day. She gives each plant a different amount of water, with the first plant getting no water and the others getting 200 mL, 400 mL, 600 mL, 800 mL, and 1,000 mL. She records the total height of the plants each day for 3 weeks.

What is the independent variable in this experiment?

Answers

Amount of water would be the independent variable in this case.

What is Independent variable?

An independent variable is a factor that you deliberately change or control to see what effect it has. Variables that respond to changes in the independent variable are called dependent variables. It depends on the independent variable. Independent variables are plotted on the x-axis.

In a scientific experiment, an independent variable is a variable that is altered or under control. It symbolizes the root cause or motivation behind a result.

The variables that the investigator modifies to test their dependent variable are known as independent variables. The dependent variable changes as a direct result of changes in the independent variable.

Therefore, Amount of water would be the independent variable in this case.

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What is visible light? Give an example and explain how the example works.

Answers

Visible light is a form of electromagnetic radiation as in radio waves, infrared radiation, ultraviolet radiation, X-rays and microwaves. Generally, visible light is defined as the wavelengths that are visible to most human eyes.


EXAMPLES:
For example, the sun produces visible light.

Incandescent light bulbs, fluorescent, and neon lights are other examples of visible light that we may see on a regular basis.

Visible light is probably the most important of all electromagnetic waves for most of us.
visible light is radiation such as radio waves

Grows and regresses (it's shed) with each monthly cycle
the vagina
the endometrium
the perimetrium
the cervix
the myometrium

Answers

pretty sure it’s endometrium?

The image shows a section of a root tip of Broad Bean with cells from the region of cell division, just behind the root cap. Five
cells have been numbered (1-5). Which statements accurately describe the mechanism by which the cells ensure that
chromosomes are passed successfully to the next generation? Select ALL that apply.
A)
Cell 1 lacks chromosomes and cannot contribute genetic material to future
cells.
Cells 4 and 5 have recently divided and have a cell plate that formed
between them
B)
c)
D)
Cell 3 is providing a mechanism for increasing genetic diversity in the
daughter cells.
Cell 2 is undergoing anaphase to separate the duplicated chromosomes to
opposite poles of the cell.
Cells 4 and 5 have recently divided and are now haploid, containing half of
a full set of chromosomes after division
E)

Answers

Answer:

The answer is B and D

Explanation:

I just did it

Answer:

b and d

Explanation:

I just did it on USA test prep

1. Smoking may damage the respiratory system because deposits from the smoke can
A. interfere with ciliary action in the trachea
B. trigger the release of antigens by the alveoli
C. block the transmission of impulses that regulate breathing
D. lower blood pressure in the mucous membranes of the bronchioles

Answers

Answer: A. interfere with ciliary action in the trachea

Explanation: I took this before and got it right.

Smoking can damage the respiratory system by damaging air sacs and alveoli.

Explanation:

Option A is correct.

The trachea is made up of ciliated pseudostratified columnar epithelium. and the smoke deposits can damage cilia and can eventually destroy them.

Cilia keep the respiratory tract clear and as they are damaged in smokers, it can lead to cough and are also at high risk of contracting pneumonia.

Option B is correct

Smoke can contribute to the aggravation of inflammatory response, contributing to the development of chronic obstructive pulmonary disease (COPD).

It can increase the release of cytokinins and trigger viral infections.

Option C is correct

Excess tobacco can block sympathetic and parasympathetic nerve impulses and can affect the normal breathing mechanism.

Option D is false

Smoking can increase blood pressure and causes hypertension.

cause of supraventricular tachycardia?

Answers

Superventricular tachycardia occurs when the system that controls your heart rhythm is not working properly.

how does mRNA help in transcription process?​

Answers

Answer:

The process of making mRNA from DNA is called transcription, and it occurs in the nucleus. The mRNA directs the synthesis of proteins, which occurs in the cytoplasm. mRNA formed in the nucleus is transported out of the nucleus and into the cytoplasm where it attaches to the ribosomes.

Explanation:

Soil formation is also called __________.
A)
compaction
B)
arcology
C)
pedogenesis
D)
tillage
E)
sedimentation

Answers

Answer:

C. Pedogenisis

Explanation:

The soil formation process is termed 'pedogenesis'. Climatic conditions are important factors affecting both the form and rate of physical and chemical weathering of the parent material.

What had a nucleus ?

Answers

Answer:

The nucleus is an organelle found in eukaryotic cells. Inside its fully enclosed nuclear membrane, it contains the majority of the cell's genetic material. This material is organized as DNA molecules, along with a variety of proteins, to form chromosomes.

In a multistep biochemical pathway, the first enzyme necessary for the synthesis of the amino acid tryptophan is inactivated with increasing levels of tryptophan and reactivated as tryptophan levels decline. This is an example of

- receptor mediated endocytosis.
- autolysis.
- irreversible damage.
- irreducible complexity.
- feedback inhibition.

Answers

Answer: Irreducible complexity

Explanation:

Irreducible complexity can be defined as characteristic of complex system it is impossible to reduce the complexity of the system by removal of any parts to maintain the functionality of the system. The given situation is an example of irreducible complexity. The increased levels of tryptophan will inactivate the synthesis process but it is again reactivated when the levels decreases this is suggestive of the fact that it is impossible to reduce the complexity of the system.

Energy resources are evenly (fairly) distributed across the Earth?
*
True
False

Answers

Answer:

False

Explanation:

Consider if there was oil everywhere then every country wouldnt have to fight for oil or go out to sea and begin drilling its simply not distrubuted evenly

What would the mRNA codon be if DNA was ATC?

Answers

the mRNA codon would be UAG

Consider the following scenario - You’ve read about how air pollution can result in acid rain and now you’re wondering how it may affect the plants in your garden.
Hypothesis: _____
Independent Variable: _____
Dependent Variable: _____
Constants: _____
Control Group: _____
Experimental Group: _____

(Please solve each for me) :0

Answers

Hypothesis; Air pollution does affect the plants in the garden.

Independent variable; Quality of air

Dependent variable; Outcome of the plants

Constants; Kind of soil and nutrients

Control groups; Plants in unpolluted environment

Experimental group; Plants in polluted environment.

What is an experiment?

When we are talking about an experiment, it has to be know that we are looking at the interplay of the variables that are involved. We are just looking at the influence of one of the variables on the other. As we change one of the variables, there is an effect also that we see on the other variable that is involved in the experimet.

The variable that we have to manipulate in the experiment is the one that we call the independent variable and as we manipulate this variable, we are going to see that there would be a change in the other variable that we call the dependent variable of the experiment.

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Which term defines a well-supported, explanation of an occurrence of a natural phenomenon?
Ο Α.
OB.
O c.
O D.
hypothesis
bias
observation
theory

Answers

Answer:

A theory.

Explanation:

A theory is an explanation that has been supported by reliable and valid evidence. It is the most well-supported explanation of an occurrence of a natural phenomenon.

I think it’s try Theory maybe

Which of the following scenarios will decrease the genetic variation of a population? (1 point)
O a mutation in a chromosome makes it more likely to undergo genetic recombination
O a population of birds suddenly decrease their frequency of sexual reproduction
O an individual works out, increasing their muscle mass
O a mutation alters the sequence of a gene resulting in a new allele

Answers

Answer:

(Q1). Which of the following scenarios will decrease the genetic variation of a population?

(answer). a population of birds suddenly decrease their frequency of se×ual reproduction

(Q2). Two of the main drivers of genetic variation are mutations and genetic recombination. During which process can these two events occur?

(answer). meiosis

(Q3). If a fish is able to breathe outside of water but does not reproduce, that trait

(answer). does not impact evolution.

(Q4). In which of the following scenarios would evolution by natural selection take place?

(answer). Penguins reproduce se×ually, producing offspring that look different from one another

(Q5). Which type of environment would tend to reduce genetic variation in a population because of evolution by natural selection?

(answer). an old growth forest with trees that are over 1,500 years old

Explanation:

these are the correct answers for the (Genetic Variation and Evolution Quick Check) 2022

The scenario that will decrease the genetic variation of a population is a population of birds suddenly decreasing their frequency of procreation.

What do you mean by Genetic variation?

Genetic variations may be defined as the presence of differences in sequences of genes between individual organisms of a species.

Mutations always lead to an increase in the genetic diversity among individuals. While decreasing the rate of crossbreading may decline the rate of genetic diversity.

Therefore, the scenario that will decrease the genetic variation of a population is a population of birds suddenly decreasing their frequency of gender reproduction. So, the correct option is B.

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What would happen if scientists used different physical characteristics

Answers

If scientists used different characteristics from the ones used then this will affect the outcome of the study and the observations made through the study will be different from the one obtained.

What are Physical characteristics?

Physical characteristic means a bodily condition or the characteristic of any person's body which is from the time of birth, accident, or any disease, or from any kind of natural physical development, or any other type of event outside the control of that individual including individual physical mannerisms, such as height, or weight of an object.

Phenotype includes an individual's observable traits, such as the height, eye color, and blood type of the person. A person's phenotype is determined by both their genomic makeup and environmental factors.

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What are 4 reasons pluto should be a planet pls help this is due today for a pbl

Answers

Answer:

The International Astronomical Union (IAU) downgraded the status of Pluto to that of a dwarf planet because it did not meet the three criteria the IAU uses to define a full-sized planet. Essentially Pluto meets all the criteria except one—it “has not cleared its neighboring region of other objects.”

Explanation:

In Darwin's thinking, the more the more closely related two different offspring organisms are related, the
more similar their habitats are.

more similar they are in size.

less similar their DNA sequences are.

more recently they shared a common ancestor.

less likely they are to have the same genes in common.

Answers

Answer:

The more recently they shared a common ancestor

• Analysis of hemoglobin proteins obtained from an individual with Sickle Cell Anemia (SCA) has been shown to contain the following amino acid sequence. Using the information from part (A), identify which of the DNA sequences is normal and which is mutated, justifying your choice.
• Sickle Cell Hemoglobin Analysis: Met – Val – His – Leu – Thr – Pro – Val
Answer-

Answers

Sickle Cell Anemia (SCA) is caused by a single point mutation in the beta-globin gene, which results in the substitution of a single amino acid in the hemoglobin protein.

Based on the amino acid sequence provided, we can conclude that the DNA sequence in the beta-globin gene is mutated, as it codes for Val at the 6th position, rather than the normal GAG.

What is the Sickle Cell Anemia about?

The normal beta-globin gene codes for the amino acid glutamic acid at the 6th position (coded for by the GAG codon), while in SCA the mutated beta-globin gene codes for valine at the same position (coded for by the GTG codon).

In the hemoglobin protein sequence provided, the 7th amino acid is listed as Val, which suggests that the corresponding codon in the beta-globin gene is GTG. This is the mutated version of the beta-globin gene that is responsible for SCA, while the normal version of the beta-globin gene would code for GAG at this position.

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I need the answers for 123

Answers

The cell membrane is a thin, semi-permeable barrier that surrounds the cytoplasm of a cell.

1) There are two of movement across the cell membrane.

2) Diffusion refers to the movement of molecules randomly, the use of energy.

3) Active diffusion refers to the movement of molecules that dash the use of energy.

Explain Diffusion.

Diffusion is the process of molecules moving from an area of higher concentration to an area of lower concentration. This process occurs naturally and does not require the use of energy. Active diffusion, on the other hand, is the process of molecules moving from an area of higher concentration to an area of lower concentration, but it requires the use of energy. Active diffusion is usually facilitated by proteins embedded in the cell membrane that act as transporters. These transporters use energy to move molecules across the membrane, either into or out of the cell.

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definition for necessary components ?​

Answers

Answer:

Necessary components are elements or ingredients that are essential for something to exist or work properly.

Explanation:

Which cellular process is the main contributor to genetic variation?

A.
independent assortment during meiosis


B.
cytokinesis at the end of mitosis


C.
production of a duplicate DNA strand


D.
asexual reproduction

Answers

Answer:

D asexual reproduction

How did the public of Russia react to the transition from a communist society to a democratic society?
a.
lack of commitment to democracy
c.
not a true understanding to democracy
b.
fully embraced democracy
d.
revolt against democracy



Please select the best answer from the choices provided


A
B
C
D

Answers

Answer:

I think it is A

Explanation:

It’s not b or d for sure

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-5 1/4 - (-7 1/2)A: -12 3/4 B: 2 3/4C: -2 1/4D: 2 1/4 Which statement best compares pioneer species with climax communities?A. Both are involved with stages of succession, but climax communities are the beginning and pioneer species are the end result.B. Both are involved with stages of succession, but pioneer species are the beginning and climax communities are the end result.C. The appearance of each indicates that the number of species in the area is increasing.D. The appearance of each indicates that the number of species in the area is decreasing. If m/KLH = 134, solve for x.19=134+2H25N(4x + 9)LK Accounts receivable turnover. (Assume that all sales are on account.) (Round your answer to 2 decimal places.) 2. Average collection period. (Use 365 days in a year. Round your intermediate calculations and final answer to 2 decimal places.) 3. Inventory turnover. (Round your answer to 2 decimal places.) 4. Average sale period. (Use 365 days in a year. Round your intermediate calculations and final answer to 2 decimal places.) 5. Operating cycle. (Round your intermediate calculations and final answer to 2 decimal places.) 6. Total asset turnover. Can muscle tightness cause back pain? 5. Jackson purchased a pack of game cards that was on sale for 22% off. The sales tax in his county is 6%. Let y represent the original price of the cards. Write an expression that can be used to determine the final cost of the cards. MUST DO ASAP PLEASE MAKE A REPLY FOR THISHello! How are you? I hope you're okay and everything. I'm fine, and already making plans for next summer. The truth is that I am happy to work at the city zoo educating other young people about the importance of protecting animal species. I think it's important to educate young people about it. Can you imagine a world without animals? There are many people who think animals are lifeless objects. I don't, and that's why I want to do something about it. Do you have any experience with a social or ecological program? Do you like to work more on social causes like unemployment, homelessness and things like that? Maybe you're like me, who likes to walk between animals and plants. Well, as I like a little of the two things, I chose the zoo; You know I like to talk a lot and make new friends. What are you going to do next summer? Already have plans? Why don't you plan to find a nonprofit to volunteer this summer? I think it would be an excellent idea and a very memorable summer. What do you think? Tell me, I want to know what you think. See you later! A cylinder shaped dispenser holds 5,652 cubic centimeters of liquid soap and is now full. The radius of the dispenser is 7.5 centimeters. What is the difference between the height of the soap in the full dispenser and the height when 4,239 cubic centimeters of soap remains in the dispenser if logx 27 +logy 4 =5 and log x 27 -logy 4=1 find x and y Plz help!!!!Select the correct answer.What testing approach does a development team use when testing the developed code rather than just the functionality of the code?A.white box testingB.black box testingC.acceptance testingD.usability testing We went to the movies last night. The line to get the tickets was athousand miles long and we thought we wouldn't get in. Mom kept sayingthat we should have bought tickets online. It worked out okay though. Wefinally got tickets and the movie was great! ASAPJust the answer pls When you are going from city w to city x, at city w, there is a milestone, after travelling for a while, you come across another milestone. How far city x from city w Which of the following is NOT a synonym of "tuanis?"o genialO criticochveretumbado Help I dont know how to do this Does anyone know this question!? Are individuals with a variation of this trait more successful at reproducing than others? it is natural tendency of bodies to eventually come to a rest position .(true or false )physic question ..................... simplify -1.5 (-3.6 +2)