HELP
Cheetah DNA is almost identical among all members of the cheetah population. This reduced genetic diversity has affected evolutionary change and limited population size. This reduction in genetic variation is best described by which of the following terms?


a. gene flow


b.genetic drift


c.gene mutation


d.natural selection

Answers

Answer 1

The correct option is B. Genetic drift

What is Genetic drift?

Genetic drift is a random process in which certain genetic traits become more or less common in a population over time due to chance events. This can happen when a small group of individuals (a founder population) becomes isolated from the rest of the population, and passes on its genetic traits to their offspring.

Genetic drift can also occur when a population experiences a natural disaster, an epidemic or a population bottleneck, which leads to the death of a large number of individuals.

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Related Questions

Why are the wings of birds and flies analogous structures?


They perform a similar function but differ in structure.

They have lost their original function.

They are similar in structure but differ in function.

They are used for a specific purpose.


Answer: B

Answers

They perform a similar function but differ in structure

To use the Hardy-Weinberg
equations, the alleles of a gene
must...
A. never be inherited.
B. follow incomplete dominance.
C. follow simple dominant or recessive inheritance.
D. be codominant.

Answers

C follow simple dominance or recessive inheritance

does replication in eukaryotic cells involve selection of multiple origins from sites licensed by the pre-replication complex

Answers

Yes, replication in eukaryotic cells involves selection of multiple origins from sites licensed by the pre-replication complex.

Replication in eukaryotic cells involves the selection of multiple origins from sites licensed by the pre-replication complex. This complex prepares the DNA for replication by binding to the DNA in the region to be replicated, and then licensing it.

This allows for the replication machinery to recognize these sites as origin sites and begin replication from them.However, it is important to note that not all of the licensed sites will be used as origin sites during replication. The selection of the actual origin sites involves additional proteins and regulatory mechanisms, which ensure that replication occurs at the appropriate time and in the appropriate locations.

These mechanisms help to prevent problems such as DNA damage or chromosomal instability.Overall, the replication process in eukaryotic cells involves a complex interplay of different proteins and regulatory mechanisms, which work together to ensure accurate and efficient DNA replication.

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which punnett square shows the results of a cross between one homozygous dominant parent and one heterozygous parent?

Answers

The Punnett square would look as follows:

B B

B BB BB

b Bb Bb

A Punnett square is a diagram used to show the possible combinations of genotypes that can result from a genetic cross. In this particular example, the Punnett square would show the results of a cross between one homozygous dominant parent (denoted as BB) and one heterozygous parent (denoted as Bb).

The Punnett square shows that all of the offspring of this cross would have a B gene (since one parent has a B gene) and half of them would have a b gene (since the other parent has a b gene). This means that the offspring would all be heterozygous (Bb).

The Punnett square can also be used to calculate the probability of each genotype occurring in the offspring. In this case, there is a 50% chance that the offspring will have two B genes and a 50% chance of being heterozygous.

Therefore, the Punnett square shown above displays the possible outcomes of a cross between one homozygous dominant parent (BB) and one heterozygous parent (Bb).

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what additional complication does a disease caused by a virus have compared to a disease caused by bacteria?

Answers

One of the additional complications that diseases caused by viruses have compared to diseases caused by bacteria is Viruses are more difficult to contain. Witch is because they are airborne and can quickly spread.

Some of the complications include:

Viruses have a high potential to mutate, which makes it challenging to treat viral diseases, while bacteria have fewer mutations.Viruses may cause dormant infections that can become active again in the future, while bacterial infections are typically more localized, and it is easier to treat.Viruses have the capacity to integrate with host DNA, which can lead to permanent changes in the host's genetic material.Viruses are more difficult to contain compared to bacteria because they are airborne and can quickly spread from one person to another through contact with infected body fluids, saliva, or blood.

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when two black heterozygous (bb ee) labrador retrievers are crossed, what phenotypic ratio is observed that differs from the typical 9:3:3:1 ratio for independently acting genes?

Answers

When two black heterozygous (bb ee) Labrador retrievers are crossed, phenotypic ratio is observed that differs from the typical 9:3:3:1 ratio for independently acting genes.

A phenotypic ratio is a numerical comparison of two phenotypes that demonstrates how frequently the occurrence of one trait corresponds with another. The phenotypic ratio produced from a test cross is used by researchers to determine the gene expression for generations of an organism.

The observed phenotypic ratios when two heterozygous (bb ee) Black Labrador Retrievers were crossed were different from the usual 9:3:3:1 ratio of independently acting genes.

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what would happen to an intact whole plant if the light reactions of photosynthesis stopped but cellular respiration continued

Answers

If the light reactions of photosynthesis stop but cellular respiration continues, the intact whole plant will experience a reduction in sugar production, but the cell will continue to respire to release energy.

When the light reactions of photosynthesis stop but cellular respiration continues, the plant will not be able to produce glucose using energy from the sun. As a result, the plant will start to break down the stored glucose that it has produced during photosynthesis. This will lead to a decrease in the plant's growth rate and the production of food.

The plant will also consume its starch reserves during respiration. As a result, the plant's leaves and other structures will gradually become weak, and the plant will not be able to maintain its structure. As a result, the plant will lose its ability to support itself, and it will eventually die.

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which group is considered a population? responses the birds of guam the birds of guam all birds and snakes of hoi polloi all birds and snakes of hoi polloi the arc-crested fruit doves of hoi polloi the arc-crested fruit doves of hoi polloi, all brown snakes on earth

Answers

A population refers to a group of individuals of the same species living in the same geographic area and potentially interbreeding. Based on this definition "The birds of Guam," is considered a population. Here option A is the correct answer.

The birds of Guam share the same species identity, and they all live in the same geographic area, providing the potential for interbreeding.

In contrast, option B, "All birds and snakes of Hoi Polloi," includes multiple species, and option D, "The arc-crested fruit doves of Hoi Polloi, all brown snakes on Earth," includes only one species of bird and one species of snake, but from different geographic locations.

Option C, "The arc-crested fruit doves of Hoi Polloi," refers to a single species, but it is unclear if all members of that species in the area are included or if they are limited to a specific geographic area.

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Complete question:

Which group is considered a population?

A) The birds of Guam.

B) All birds and snakes of Hoi Polloi.

C) The arc-crested fruit doves of Hoi Polloi.

D) The arc-crested fruit doves of Hoi Polloi, all brown snakes on Earth.

9. The food web for an ecosystem is represented below. (7.5B) Rabbits Foxes Mice & Carrots Owls Birds Grasshoppers, √ Grasses Gr Grains Based on the food web, the populations of which two organisms would directly impact the population of grasshoppers? O O O O Carrots and owls Birds and grasses Mice and grains Foxes and mice​

Answers

Birds and grasses because birds eat grasshoppers and grasshoppers eat grass

Explain the negative impact of deforestation, and also 2 ways Humans have made efforts to protect our planet's forestland in a short paragraph (4 or more sentences).

Answers

Answer:

See below.

Explanation:

Deforestation, the process of cutting down and clearing forests for other land uses, has numerous negative impacts on the environment. It contributes to climate change by releasing large amounts of carbon dioxide into the atmosphere, as trees absorb carbon dioxide and produce oxygen. Deforestation also leads to soil erosion, loss of biodiversity, and increased frequency and severity of natural disasters like floods and landslides. Additionally, deforestation destroys the habitats of countless species, which can lead to their extinction.

Fortunately, humans have taken steps to protect our planet's forestland. One way is through reforestation efforts, which involve planting new trees in areas where forests have been cleared. Another way is through conservation, which involves protecting existing forests and preventing further deforestation. Governments and environmental organizations have established protected areas and implemented policies and regulations to limit deforestation and promote sustainable land use practices. Additionally, there is a growing movement to promote the use of sustainable products, like recycled paper and wood products from sustainably managed forests, which can help reduce the demand for new products that contribute to deforestation.

Ms. Tanager had two units of blood transfused into a vein, or _______venously.

Answers

Ms. Tanager had two units of blood transfused into a vein, or venously.

What is blood transfusion?

Blood transfusion is a medical therapy that involves transferring blood or blood components from one person (the donor) to another (the recipient). The technique is usually used to replace blood lost as a result of injury or surgery, as well as to treat certain conditions that result in a shortage of blood cells, such as anemia, hemophilia, and blood clotting disorders.

How is blood transfusion done?

Blood transfusions are generally done in a hospital or clinic by a qualified medical professional. The transfusion is usually done intravenously, meaning that the blood is injected into the patient's vein using a needle or a catheter. It's critical that the needle is put into the correct vein and that the needle remains in place for the duration of the transfusion. It's also critical that the blood is given at the correct rate, which is usually between 2 and 4 hours for a typical transfusion.

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which type of anterior pituitary cell secretes adrenocorticotropic hormone (acth)? a. thyrotrophs b. corticotrophs c. somatotrophs d. gonadotrophs e. lactotrophs

Answers

The correct answer is B. Corticotrophs are a type of anterior pituitary cells that secrete Adrenocorticotropic Hormone (ACTH).

Corticotrophs are a type of endocrine cell in the anterior pituitary gland that produces and secretes several hormones, including ACTH. ACTH, in turn, stimulates the production and release of cortisol from the adrenal glands, which plays an important role in regulating the body's stress response and metabolism. So this hormone is released when the body is under stress, and it helps to regulate metabolic processes such as metabolism, immune response, and electrolyte balance. Corticotrophs are also involved in the regulation of other hormones such as growth hormone and thyroid-stimulating hormone.  

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ouabain is a substance that has been used as an arrow poison and it inhibits sodium-potassium atpase. if applied to a neuron, what is the likely effect of the ouabain on action potentials?

Answers

Ouabain is a chemical used on poison arrows in Africa. It works by inhibiting the Na+/K+ pump. This have on an action potential effect there would be no direct effect because the pump is used to maintain ion distributions, not to cause an action potential.

Ouabain is a toxic steroid compound that is found naturally in certain plants, such as the African arrow poison plant. It has been used historically in traditional medicine as a treatment for a range of conditions, including heart failure, but its use is now limited due to its toxic effects.

Ouabain acts by inhibiting the sodium-potassium pump, a vital membrane protein that maintains the ionic balance in cells. This results in an accumulation of intracellular sodium and a depletion of intracellular potassium, leading to a variety of physiological effects, including increased cardiac contractility and heart rate, as well as vasoconstriction. In addition to its effects on the cardiovascular system, ouabain has also been shown to have effects on the central nervous system, including the regulation of mood and behavior.

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Complete Question:-

Ouabain is a chemical used on poison arrows in Africa. It works by inhibiting the Na+/K+ pump. What effect would this have on an action potential?

in prokaryotes, in which direction are hydrogen ions pumped by the electron transport system of photosynthetic membranes?

Answers

The electron transport system of photosynthetic membranes in prokaryotes pumps hydrogen ions outwards from the stroma towards the thylakoid lumen, creating an electrochemical gradient which is used to generate ATP from ADP.

This process is known as photophosphorylation and is an essential step in the light-dependent reaction of photosynthesis.

The electron transport system of photosynthetic membranes in prokaryotes pumps hydrogen ions outwards from the stroma, towards the thylakoid lumen. This process is known as photophosphorylation, and occurs during the light-dependent reaction of photosynthesis.


During photophosphorylation, light energy is used to excite electrons within the pigment molecules in photosystem II. The excited electrons are transferred along the electron transport chain and eventually reach photosystem I. The electrons are then donated to the electron acceptor NADP+ (nicotinamide adenine dinucleotide phosphate), resulting in the formation of NADPH.


During this process, hydrogen ions are pumped out of the stroma and into the thylakoid lumen. This creates an electrochemical gradient, known as the proton-motive force, which is used to generate ATP from ADP. This is known as chemiosmosis, and is the final stage of the light-dependent reaction of photosynthesis.

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describe the relationship between the number of colonies observed and the likely mutation rate of the strain.

Answers

The relationship between the number of colonies observed and the likely mutation rate of the strain is an inverse one. As the number of colonies observed increases, the likely mutation rate decreases, and vice versa. This is because the more colonies that are observed, the more likely it is that the strain has experienced a selection pressure, which makes it less likely that it has undergone mutations.

Conversely, a strain with fewer colonies is more likely to have experienced mutations due to the decreased selection pressure.
It is important to note that the number of colonies observed is not the only factor in determining the mutation rate of a strain.

Other factors, such as the specific environment in which the strain was grown, the strain’s genetic makeup, and the presence of additional agents, may all play a role in influencing the mutation rate of a given strain.

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the immigrant lineage has only a population of 20 while the native lineage 280 birds sampled. what event on the island reduced the overall diversity of this group? how might the size of the gene pool contribute to the low variety in beak size for the immigrants?

Answers

The size of the gene pool contributes to the low variety in beak size for the immigrants by being smaller in size and leading the species to decrease or get extinct.

The group's overall variety would have declined during a drought. The immigrants' tiny gene pool adds to the lack of variation in beak size since under these conditions, populations are more likely to decline quickly or die extinct.

Varieties are the minute adjustments that various members of the same species make. Different genetic information (DNA) is the primary cause of many of these variations. The term "gene pool" refers to the diversity of DNA inside a species. The likelihood of survival increases with the size of the gene pool. Often times, these minute variations give some creatures an advantage. This increases the likelihood that they will live and have children with these characteristics.

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defects in cell signaling can lead to a cancerous cell (a cell that grows and divides in an uncontrolled manner). this could be the result of:

Answers

Defects in cell signaling are the result of a mutation or abnormality in one or more genes that regulate cell division and growth which leads to a cancerous cell.

What is a cancerous cell?

A cancerous cell is a cell that grows and divides uncontrollably due to defects in cell signaling. A mutation or abnormality in one or more genes that regulate cell division and growth can lead to the development of cancerous cells. As a result of these abnormalities, cells begin to divide and grow uncontrollably, leading to the development of tumors and cancer.

In normal cells, cell signaling pathways control the cell cycle and ensure that cells divide and grow in a regulated manner. These pathways include numerous signaling molecules and proteins that communicate with each other to control cell growth, division, differentiation, and survival.

In cancerous cells, defects in these signaling pathways cause uncontrolled cell division and growth, leading to the development of tumors and cancer.

The types of defects in cell signaling that can lead to cancerous cells include mutations in oncogenes or tumor suppressor genes, alterations in the expression of signaling molecules, and changes in the activity of signaling proteins. These defects can be caused by genetic factors, environmental factors, or a combination of both.

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the layer of connective tissue that surrounds the outside of skeletal muscle (i.e., just below the fascia) is called the

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The layer of connective tissue that surrounds the outside of skeletal muscle , just below the fascia is called the Epimysium.

Epimysium is a thin layer of tissue that surrounds skeletal muscles, separating them from surrounding tissues and organs. The layer of connective tissue that surrounds the outside of skeletal muscle (i.e., just below the fascia) is called the Epimysium.

The term "muscle fascia" refers to the layer of connective tissue that covers and holds together the muscle bundles.

Each muscle is covered in connective tissue that splits the muscle into smaller segments called fascicles.

It helps to facilitate muscle movements while also providing structural support to the muscle.

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which type of leukemia happens as a result of myeloblasts that are well differentiated and has a slow onset?

Answers

The type of leukemia that occurs as a result of well-differentiated myeloblasts and has a slow onset is Chronic myeloid leukemia (CML).

Chronic myeloid leukemia (CML) is a kind of blood cancer that affects bone marrow's myeloid cells. The overgrowth of these cells in the bone marrow and bloodstream causes CML, which can cause a variety of symptoms. The cancer slowly progresses over time and affects all aspects of the body.

CML's most prevalent sign is a high white blood cell count. The leukemic cells in CML are similar to, but not quite the same as, normal white blood cells. They develop more slowly than acute leukemia cells and have a longer life span in the blood than normal white blood cells.

Treatment for CML has progressed dramatically over the years, and patients with the disease can now live for many years.

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Changes in single neuron firing rates in area V1 of monkeys as a function of selective attention would provide support for________.a. early-selection models of attentionb. capacity limitation models of attentionc. computational models of attentiond. late-selection models of attention

Answers

Changes in single neuron firing rates in area V1 of monkeys as a function of selective attention would provide support for the computational models of attention. The correct option is (C).

Attention is the cognitive process that allows humans to concentrate on one specific aspect of their surroundings, whether it is a sensory input or a thought in their minds.

The attentive system can be directed internally or externally. Several models have been developed to explain the mechanisms of attention.

The two primary categories of models of attention are early-selection models and late-selection models.

The computational model of attention is a theoretical model that has emerged from cognitive psychology and cognitive neuroscience research.

It proposes that selective attention is not the result of a bottleneck or filter, but rather a selection process that includes a combination of selective amplification of relevant information and selective inhibition of irrelevant information.

In addition, it proposes that attentional selection is influenced by previous experience, task demands, and the individual's goals.

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What antibodies are involved in humoral immunity?

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Humoral immunity involves the production and activity of antibodies, which are proteins produced by B cells (a type of white blood cell) that recognize and bind to specific antigens (foreign substances) on the surface of pathogens such as bacteria, viruses, and fungi.

The antibodies are then able to neutralize or eliminate the pathogens by various mechanisms such as blocking their ability to enter host cells, marking them for destruction by other immune cells, or activating complement proteins to destroy them directly.

There are five major classes (or isotypes) of antibodies that can be produced by B cells, each with a distinct structure and function: IgM: the first antibody produced during an immune response, primarily found in the bloodstream and able to activate complement proteins.

IgG: the most abundant antibody in the bloodstream, able to cross the placenta and provide passive immunity to the developing fetus.

IgA: found in high concentrations in mucosal secretions such as saliva, tears, and breast milk, providing protection against pathogens at the body's entry points.

IgD: found on the surface of B cells and involved in B cell activation.

IgE: involved in allergic reactions and defense against parasites.

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examine the figure of a human nephron. where and when does osmolarity of the filtrate increase? a human nephron. it consists of the glomerulus, bowman

Answers

The osmolarity of the filtrate increases at the proximal convoluted tubule in the human nephron.

Osmolarity refers to the concentration of dissolved particles or solutes in a solution. This concentration determines the movement of fluids across cellular membranes, as well as the movement of solutes within the solution. In the human body, osmolarity is critical in regulating water and electrolyte balance, as well as aiding in the filtration of blood and the production of urine.

The human nephron is the functional unit of the human urinary system, which is responsible for the filtration of blood and the regulation of fluid and electrolyte balance. It is responsible for filtering blood, regulating fluid and electrolyte levels, and producing urine. The nephron accomplishes this through a complex process of filtration, reabsorption, and secretion that occurs in several distinct regions of the nephron.

In summary, the osmolarity of the filtrate increases at the proximal convoluted tubule in the human nephron.

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deoxygenated blood flows from the right ventricle to the left ventricle. what defect does this most likely describe?

Answers

The defect described in the question is most likely a Ventricular Septal Defect (VSD), a hole between the right and left ventricles of the heart, which allows deoxygenated blood to flow from the right ventricle to the left ventricle. Treatment depends on the size and type of the VSD, and can range from medications to surgery.

This most likely describes a defect called a Ventricular Septal Defect (VSD). A VSD is a hole between the right and left ventricles of the heart, which allow deoxygenated blood to flow from the right ventricle to the left ventricle. This can cause a wide variety of symptoms, ranging from heart murmurs, fatigue, and difficulty breathing, to a bluish skin color (called cyanosis). VSDs can be classified as large, moderate, or small, depending on the size of the hole. Treatment may involve medications, surgery, or a combination of the two.

Ventricular Septal Defects can be present at birth (congenital) or develop later in life (acquired). Congenital VSDs can be caused by genetic factors, complications with the mother during pregnancy, or environmental factors. Acquired VSDs are usually caused by diseases that affect the heart, such as rheumatic fever.

Diagnosis of VSD is usually done through imaging techniques, such as echocardiogram and cardiac MRI. Treatment depends on the severity and type of the VSD. For small or moderate defects, medications, such as diuretics and beta blockers, may be used to reduce symptoms. For large or complex defects, surgical repair may be necessary.

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which labeled cell in the diagram secretes a hormone that stimulates events that increase blood glucose concentration?

Answers

The labeled cell in the diagram that secretes a hormone that stimulates events that increase blood glucose concentration is cell "B" (Beta cells).

Beta cells are found in the islets of Langerhans in the pancreas and secrete the hormone insulin, which promotes the uptake and storage of glucose by cells in the body, thereby decreasing blood glucose concentration. However, if blood glucose levels drop too low, another hormone called glucagon is secreted by alpha cells in the pancreas, which stimulates the liver to break down stored glycogen and release glucose into the bloodstream, thereby increasing blood glucose concentration. So, cell "A" (Alpha cells) would be responsible for secreting the hormone that stimulates events that decrease blood glucose concentration.

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a transgenic organism is one that a transgenic organism is one that changes gender over time. provides gene information to another organism. has received genetic information from another organism. changes gene frequency over time.

Answers

A transgenic organism is one that has received genetic information from another organism.

The genetic information in transgenic organism can come from the same species or a different species, and it results in a change of gene frequency over time. In other words, a transgenic organism's DNA is altered from its original state by the introduction of foreign genetic material. This alteration can lead to a change in the organism's phenotype, or physical characteristics.

The purpose of making transgenic organisms is to enable organisms to acquire certain traits that were not previously possessed. So that the transgenic organisms in the form of food crops will be resistant to pests, or improve the quality of the plants.

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what was the effect of tsh on the normal rate's metabolic rate? how does it compare to the rnomal rate's metabolic rate

Answers

TSH has an effect on the normal rate's metabolic rate and it can either increase or decrease depending on the amount of TSH present in the body. An increase in TSH leads to an increase in metabolic rate while a decrease in TSH leads to a decrease in the metabolic rate.

The thyroid stimulating hormone (TSH) affects the metabolic rate of the body. TSH stimulates the thyroid gland to produce thyroid hormones which in turn increase the metabolic rate. An increase in the metabolic rate leads to an increased breakdown of carbohydrates, fats, and proteins in the body. The rate of metabolism is determined by the number of thyroid hormones present in the body. In a normal person, the amount of TSH and thyroid hormones remain at a stable level, resulting in a stable metabolic rate.

However, when there is an increase in the amount of TSH, the metabolic rate of the body increases and this is known as hyperthyroidism. This increase in the metabolic rate can lead to a variety of symptoms like weight loss, palpitations, sweating, and heat intolerance. On the other hand, when the amount of TSH decreases, the metabolic rate of the body decreases and this is known as hypothyroidism. Symptoms of this condition include weight gain, fatigue, dry skin, and cold intolerance.

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Describe what is happening in the diagram below*
DNA
Human cell
DNA
Section of human DNA
Bacterial cell
1 point
Bacterial DNA
Bacterial cell
A human cell is getting bacteria to be able to make antibodies against the bacteria.
O A hybrid organism with 50% bacteria DNA and 50% human DNA is being made.
A human gene is being inserted into the bacteria's genome so it can make a
human protein for medical use.
A bacterial cell is getting its DNA removed and then cut with restriction enzymes.
Afterwards, it is put into a human nucleus.

Answers

Answer:

The bacterium

Explanation:

antibodies + artificial dna + Recombinant DNa+restriction enzyme= DNA

whether the effect of a neurotransmitter is excitatory or inhibitory depends on group of answer choices

Answers

To the effect of a neurotransmitter is excitatory or inhibitory is: The effect of a neurotransmitter on a neuron can either be excitatory or inhibitory depending on the type of receptor it binds to.

Excitatory neurotransmitters, such as glutamate and aspartate, bind to and activate ligand-gated ion channels, resulting in an influx of sodium ions, depolarizing the membrane and triggering an action potential.

Inhibitory neurotransmitters, such as gamma-aminobutyric acid (GABA) and glycine, bind to and activate ligand-gated ion channels, resulting in an influx of chloride ions, hyperpolarizing the membrane and preventing an action potential.

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on the cellular level, how is gastrulation accomplished in echinoderms, amphibians, and birds? in general terms what does gastrulation accomplish?

Answers

Gastrulation in echinoderms, amphibians, and birds is accomplished through the invagination of different cells.

In general, gastrulation is the process that reorganizes cells to form the three germ layers, which are necessary for the further development of an organism.

Gastrulation is the process in which cells rearrange to form the three germ layers: the ectoderm, mesoderm, and endoderm.

In echinoderms, gastrulation is accomplished through the process of archenteron formation, which is when the mesoderm forms from the invagination of cells from the surface of the embryo.

In amphibians, gastrulation is accomplished through blastopore closure, which is when the opening at the blastula stage of the embryo closes.

In birds, gastrulation is accomplished through the formation of the primitive streak, which is when the ectoderm folds and inwards to form a groove-like structure.


In summary, gastrulation is the first step of morphogenesis, the development of form and structure, which will determine the shape of the organism. The three germ layers will further differentiate and develop into the organs, tissues, and cells that make up the organism.

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when dna probes are used to identify bacterial dna similarities by hybridization, the probe dna is heated and the template dna is treated to separate the 2 strands. why would the probe dna be heated?

Answers

Answer:

The probe DNA is heated to denature or separate the two strands of the double-stranded DNA to make it single-stranded. This is because hybridization, the process of joining two complementary strands of DNA, can only occur between two single-stranded DNA molecules. By heating the probe DNA, it denatures and becomes single-stranded, allowing it to hybridize with the target DNA under specific conditions. The temperature at which the denaturation or melting occurs depends on the base composition of the DNA, specifically the amount of G-C pairs. This is known as the melting temperature or Tm.

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