Homologous recombination can be used in the lab to replace a gene of interest with a selection marker. To confirm that the selection marker is integrated into the correct place in the genome, sometimes a negative selection is used in addition to the standard positive selection marker. Which of the following would act as a negative selection marker?

a. Transformation of a different gene, which does not act as a selection marker, to replace the gene of interest.â
b. A second marker in the knock out cassette, that if inserted into the genome results in cell death when plated on selective media.
c. Transforming the cells with two different knock out cassettes, each containing the same selection marker3. â

Answers

Answer 1

Answer:

b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.

Explanation:

General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the locus/loci of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.


Related Questions

definition for artificial language​

Answers

Answer:

An artificial language is a language that has been created by humans, as opposed to a natural language, which is a language that has evolved naturally over time. Artificial languages are typically constructed to be used as a means of communication between humans, although they can also be used for other purposes, such as computer programming.

Explanation:

¿Cuál célula es capaz de sobrevivir en
condiciones extremas? *

A) Eubacterias
B) Procariotas
D) Eucariotas
E) Arqueobacterias​

Answers

Answer:

E.) Arqueobacterias​

Explanation:

can someone answer this for me please

Answers

Answer:

The constants: 14 hours of light per day; food one time per day 5 oz; wheel for exercise; cage size

The independent variable: the temperature

The dependent variables: length and weight

Data to be collected: weight of mice; length of mice

A flaw in the experiment: the sample size is to small to be random; it doesn't specify a control group

Explanation:

The constants are the controlled variables. The scientist controls these items because a variable that remains unchanged prevents it from having any effect on the outcome.

The independent variable is the condition manipulated by the scientist to see its effects.

The dependent variables are the ones being measured or tested for in an experiment.

Randomization means every subject gets an equal chance at being in the control group. There are to few subjects. A control group would consist of elements that present exactly the same characteristics of the experimental group, except for the variable applied to the latter.

Which term best describes the pattern of distribution for the human
population shown in the picture?
O A. Random
B. Sparse
C. Even
D. Clustered

Answers

Answer:

Its B. Sparse

Explanation:

i just took

Population is the number of species in the given area. The image shows sparse pattern of distribution for the humans, i.e., option B.

What is sparse pattern of distribution?

A sparse population is the area where few people resides. In such a case, the area does not densely populated. Their temperatures is colder and have few buildings.

The image here as shown represents sparse pattern of population distribution.

Thus, the correct option is B.

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The Arg genes that encode the enzymes for arginine biosynthesis are located at several positions around the genome of E.coli, and they are regulated coordinately by a transcription regulator encoded by the ArgR gene. The activity of the Arg R protein is modulated by arginine. Upon binding arginine, ArgR alters its conformation, dramatically changing its affinity for the DNA sequences in the promoters of the genes for the arginine biosynthesis. A. Given that ArgR is a repressor protein, would you expect that ArgR would bind more tightly or less tightly to the DNA sequences when arginine is absent?

Answers

Solution :

The Arg genes is used to synthesize the arginine. Arginine is chemical building block. It is very important and is used by our body to make the proteins for our body. When the Arg ID is abundant, the expression for the biosynthetic genes must turned off. And if the ArgR behaves as the gene repressor, then the binding of the arginine increases the infinity for the regulatory sites, and allows it to the bind and then shut off the expression of the genes.

If the ArgR acts as the gene activator, then the binding of the arginine would be predicted to reduce the affinity for the regulatory DNA, and preventing the binding and the shutting off the expression for the Arg genes.

state two types of water​

Answers

Answer:

Mineral water and Distilled water

That is the table pls help

Answers

Abena is the smallest Elephant. Chipo is the largest Elephant. The increase in shoulder height of Bisa over 15 years is 85 cm.

How do elephants develop and grow?

Early in their second decade of life, elephants become sexually mature. African elephants reach sexual maturity between the ages of 10 and 12, while Asian elephants reach sexual maturity around the age of 14.

How much time does it take an elephant to grow up?

Total Size. Physical and emotional development in elephants takes a very long time. The majority of their size is reached by the age of 15, but they frequently continue to gain weight and develop in size until they are around 20 years old.

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how do i scientifically defend the theory of evolution

Answers

Answer:

wait wdym defend? like you're in an argument about the theory of evolution and you want to defend your point........?

The length of a DNA double helix increases by 0.34 nm (nanometres) for every pair of nucleotides. The total number of nucleotides in a human body cell is
[tex]1.2 \times {10}^{10} [/tex]

Calculate the total length of a double helix in a human body cell (answer to be given in meters)

Pls give me a quick answer​

Answers

(0.34 x 10^-9)(1.2 x 10^10) =4.08m

LIMITED TIME, 20 MINS HELP!!!!! ASAP!!!!
Using electricity sparingly in your home is beneficial for the environment because it:

Select one:

1. increases the amount of greenhouse gases emitted into the
atmosphere.

2. increases the rate of plant growth

3. increases levels of oxygen in the atmosphere.

4. reduces the amount of greenhouse gases emitted into the
atmosphere.

Answers

Answer:

As you improve the energy efficiency of your home, you need less electricity and thus rely less on carbon-intensive power plants. This reduces your home’s demand from the plant, which in turn benefits the environment by reducing their carbon dioxide emissions.

4. reduces the amount of greenhouse gases emitted into the atmosphere.

This system provides nutrients for cells in all tissues and organ systems.

A nervous
B. muscular
C. Digestive
D. respiratory​

Answers

Answer:

the correct answer is option C. the digestive system

The system provides nutrients for cells in all tissues and organ systems is option C. Digestive as it breaks downs the food into simple forms used as nutrients.

The digestive system is an organ system that converts foods an organism eats into their simplest forms that are glucose makes carbohydrates, amino acids make up protein, and fatty acids make up fats.

The nutrients or simple forms of food are absorbed into the bloodstream from the small intestine. It begins at the mouth and ends at the anus.various digestive organs make the digestive system such as mouth, stomach, large and small intestine with the pancreas, bile duct, and liver.The circulatory system takes the absorbed nutrients to various organs and cells.

Thus, the correct answer is - C. Digestive system.

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Why is purification of water and nutrient salts so important in studies involving mineral nutrition using hydroponics?​

Answers

Answer:

Hello!!!

Explanation:

Aish hi hona tum?

The disadvantage of batch fermentations, as opposed to continuous feed systems, is that

Select one:
a. new substrate must be added to the fermentor continuously for the fermentation to continue.
b. there is a limit to the amount of product formed since all materials are added to the fermentor at the beginning.
c. it is small scale production of a product.
d. the end product is not as pure as the product made by the continuous feed process.

Answers

Answer:

according to me may be option number b

Discuss the possible theories
around water uptake in plants
Suggest the best and most
acceptable one..
and

Answers

Answer:

There are several theories around water uptake in plants, each with their own strengths and limitations.

One theory is that water is taken up by plants through osmosis, which is the movement of water from a region of high concentration to a region of low concentration. According to this theory, plants absorb water from the soil through their roots, and the water moves across the cell membrane into the plant cells by osmosis. This theory is supported by the fact that plants have a higher concentration of solutes inside their cells than in the soil, which creates a concentration gradient that drives the movement of water into the plant.

Another theory is that water is taken up by plants through transpiration, which is the loss of water vapor from the leaves of plants. According to this theory, plants absorb water through their roots and transport it to the leaves, where it is lost through the stomata (pores) on the surface of the leaves. This theory is supported by the fact that plants can lose significant amounts of water through transpiration, and that the rate of transpiration is influenced by factors such as humidity and temperature.

A third theory is that water is taken up by plants through capillary action, which is the movement of a liquid through a narrow tube or channel. According to this theory, plants absorb water from the soil through their roots and transport it to the rest of the plant through the xylem (water-conducting tissue) by capillary action. This theory is supported by the fact that the xylem of plants has a small diameter and a high surface tension, which allows it to transport water by capillary action.

Overall, the most acceptable theory around water uptake in plants is a combination of osmosis, transpiration, and capillary action. All three mechanisms play a role in the uptake and transport of water in plants, and it is likely that different mechanisms are more or less important depending on the specific plant and the environmental conditions.

Explanation:

HELP LIMITED TIME ASAP!!!!!!!!!!!!!!
Which greenhouse gas do humans release into the atmosphere the
most?

Select one:
1, Nitrogen

2, Water Vapor

3, Oxygen

4, Carbon Dioxide

Answers

Answer: Carbon dioxide

Explanation:Because,Carbon dioxide from the burning of fossil fuels is the largest single source of greenhouse gas emissions from human activities.


What effect would a series of major volcanic eruptions have on global climate change?

A) the increase in atmospheric particles with increased range globally

B) The blanket effect of Ash would increase temperatures globally

C) atmospheric ash circulation would cause extreme temperature fluctuations and rainfall

D) Ash clouds would reflect the suns energy and decrease temperatures globally

Answers

The effect that a series of major volcanic eruptions would have on global climate change would be Ash clouds would reflect the sun's energy and lower temperatures globally.

What is volcanic winter?

A volcanic winter is the reduction in temperature caused by volcanic ash and sulfuric acid droplets obscuring the sun, usually after a volcanic eruption. Long-term cooling effects are primarily dependent on the ejection of sulfide compounds in aerosol form into the upper atmosphere.

With this information, we can conclude that a major eruption would cause a volcanic winter, with a reduction in temperatures globally.

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What process occurs when molecules move from a crowded area to a less crowded area ?


Show/tell me the EVIDENCE

Answers

It’s called diffusion, it’s the random motion of particles from high areas to low areas.

Cell wall hut not cell
,
Robert Hooke discovered
Organelles.​

Answers

Answer:

Explanation:

WHAT IS YOUR QUESTION ??

Robert hooke discovered cell wall but not the cell organelle because the cell organelle is much smaller in size as compared to the cell wall. Explanation: The microscope which was used by the scientist Robert hooke was a simple microscope

Human activity can contaminate water resources by introducing excess ____, such as nitrogen and phosphorous found in______ and human waste.

Answers

Answer:

minerals and industrial.

Explanation:

Human activity can contaminate water resources by introducing excess minerals such as nitrogen and phosphorous found in industrial and human waste. The agricultural activities such as applying fertilizer and pesticides pollute the water bodies while on the other hand, human waste which is not properly dispose pollute the environment and water bodies that has adverse effect on the environment and its organisms that are present in it.

Anya is a veterinary scientist who works in a lab that is attempting to develop better treatment against snakebites. She is currently setting up an experiment that involves running a current through the venom to see if that will separate the particles inside. In the past, this has not worked, but Anya has high hopes. If you were to read the research paper that Anya writes after the experiment, which would MOST likely be the title?

A.
Exploring Chemicals that Combat Viperine Venom

B.
Results of Shock Therapy on Elapine Venom Samples

C.
Discussing the Quality of Antivenin Serums on the Market

D.
Using Radiation to Dissolve Elapine Venom

Answers

Answer: B

Explanation:

Q1: what is sleet?

Q2: how big can raindrops get?

Answers

Q1. It is a form of precipitation that consists of ice pellets often mixed with rain or snow. it is usually soft and contains some traces of ice. It can be dangerous to drive with this on the road.

Q2: They can get as big as 4 millimeters. rain drops don't usually get any bigger without seperating into two droplets.

Explanation:

(I hope this helps! Have a great day!)

what is codon, and what does it represent in protein synthesis?​

Answers

Answer:

A codon is a sequence of three DNA or RNA nucleotides that corresponds with a specific amino acid or stop signal during protein synthesis. Each codon corresponds to a single amino acid (or stop signal), and the full set of codons is called the genetic code.

Explanation:

Hope you have a great day

Two objects are described below.
Thing 1: It has a mass measured by a pan balance.
Thing 2: It takes the shape of a container.
Which statement is true?
O Both objects are matter.
O Both objects are non-matter.
O The first object is matter, and the second object is non-matter.
The first object is non-matter, and the second object is matter.

Answers

Mass is measured by balance and weight is measured by balance.

Is mass measured by Pan balance?

Two pans are located on either side of a lever in the pan balancing scale. To achieve balance and determine the weight of the other thing, weights are placed on one side of the object and on the other side. Typically, the mass is expressed in grammes or kilogrammes.

Because of its practicality and accuracy, comparative weighing is frequently used to compare two things' masses rather than their absolute values. When preparing reagents and stock solutions, a top pan balance is typically used for rough weighing because minor weight differences do not significantly affect the outcome of analysis.

Two pans are integrated into this particular laboratory scale on either side of a lever. It has two separate applications. 

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When Thing 1: It has a mass measured by a pan balance. Thing 2: It takes the shape of a container. Then Both objects are matter statement is true.

What is Matter?

Any substance with mass and which has volume is considered matter because it occupies space.

Properties of matter:Any characteristics that can be measured, such as an object's density, color, mass, volume, length, malleability, melting point, hardness, odor, temperature, and others, are considered to be part of its properties.Mass:A body's mass is an inherent quality. earlier before  the discovery of the atom and particle physics, it was widely considered to be tied to the amount of matter in a physical body.

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i need help plz i’ll give you brainilest

Answers

the answer is Available habitats
The answer is all of those

Wetlands and estuaries purify water and filter out...

A dead organisms
B salt
C pollution
D waste products

Answers

Answer:

C. Pollution

Explanation:

Wetlands and estuaries purify water and filter out pollutants.

13. (a) Define area and state its SI units? ​

Answers

Answer:

Area is defined as the magnitude of product of length into breadth.

S. I unit is in m2

Explanation:

A= L x B

What is described: Blood type AB

pls help me i need to finish this biology test tonight

Answers

Answer:

The AB blood type is one that, according to the blood type classification, does not have Anti-A or Anti-B antigens in its cells, with which, in short, it is constituted as the only blood type that is a universal receptor in case you need a transfusion, since you can receive blood from people with blood type 0, A, B or the same AB.

period when cell is not engaged in division

Answers

Interphase is the period of cell cycle in which the cell is not engaged in any kind of cell division. This phase is marked as the preparatory phase for the actual cell division.

What is Cell cycle?

The cell cycle is a series of steps during which the chromosomes and other cellular material double to make two copies of the genetic material. The cell then undergoes division to form two daughter cells, each of the daughter cell receiving one copy of the doubled chromosomal material. The cell cycle is complete when each of the daughter cell is surrounded by its own outer membrane.

   

The interphase is the period when the cell is in a non-dividing state of the cell and this phase can be in different stages: the first gap (G1) between the last mitosis (M) and the S-phase (phase of DNA synthesis) and the second gap (G2) phase between the completion of the S-phase and the next mitosis (M).

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4. If the mass of two objects increases and the
distance between them stays the same, what happens to the gravitational force
between the two objects?

A. It decreases.
B. It remains the same.
C. It fluctuates.
D. It increases.

Answers

Answer:

As the mass of either object increases, the force of gravitational attraction between them also increases.

How do the locations where photosynthesis occurs differ in prokaryotes and eukaryotes?

Answers

Answer:

In eukaryotes, photosynthesis occurs in the chloroplasts of the cell. However, prokaryotes do not have membrane-bound organelles so they don't have chloroplasts. In prokaryotic cells, photosynthesis occurs in the infoldings of the cell membrane, which contain chlorophyll.

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Refer to the two paragraphs beginning onpage 3 and ending at the top of page 4.Which premise do these two paragraphs MOSTsupport? Alexis deposited $6,000 into an account that pays 3% interest compounded annually. He doesnt withdraw or deposit any money for 4 years. How much interest did he earn in that time? Which of the following information is not required when facilitating a release of information request or authorization Chris received an email about his favorite band going on tour. He was happy to learn that tickets were only $35 apiece. However, once online, he soon realized that he also had to pay for parking at the venue, a service charge, tax, and a convenience fee. His ticket ended up costing him $85. The email Chris received used which of the following persuasion technique Explain how the following two processes alter the percentage of carbon dioxide in the atmosphere (1) combustion HHHHHHH-HHHHHHHHHWhat best describes these three molecules?O They are not isomers.O They are structural isomers.O They are geometric isomers.They are both structural and geometric isomers. If some one makes and many slices of toast of toast as possible in 4 minutes and 40 seconds how many slices they can make? True or false: Minor acts of dishonesty and slight deviations from the truth are harmless and should be encouraged in business. PSA IF YOU ARE GOOD AT ECONOMICS PLEASE REPORT AND ANSWER MY QUESTION!!! THANKSSS. Marketers must determine the price of a product carefully on the basis of the potential buyer's beliefs about O the product's new advertising campaign. O its value. O the environment. O the economic outlook. O the cost to manufacture the product. An office manager is planning to set up three computer workstationsand a file cabinet in a space that is 27 feet wide. The file cabinet takes up3 feet. What is the width of the space available for each computer workstation?PLEASE HELP Elena paints the back of the second aquarium it has a height of 1 3/4 feet the painted area is 5 5/6 square feet what is its length am is paying off his eight-year, $15,360 loan in semiannual installments. The loan has an interest rate of 9.58%, compounded semiannually, and a service charge of $1,294.64. Once the loan has been fully paid off, what percentage of the total finance charge will the service charge be? Round all dollar values to the nearest cent.a.5.48%b.8.43%c.18.55%d.15.65%Please select the best answer from the choices providedABCD . Two spherical objects have masses of 8,000 kg and 5.0 kg. Their centers are separated by a distanceof 1.5 m. Find the gravitational attraction between them. Suppose we have a disease phenotype that is determined by a single gene with two alleles. Further suppose it follows a autosomal recessive mode of inheritance with reduced penetrance. We have an individual who is unaffected (does not have the disease phenotype) and whose genotype is unknown. We cross this individual with a true-breeding affected individual. How many possible genotypes can this unaffected individual have at the disease locus Please Help I Didn't Understand The Lesson Solve using the quadratic formula. Show all work. Write each solution in simplest form. No decimals. 2. What are the domain and range for the exponential parent function,where b is a positive real number not equal to 1 and a > 0? Write the equation for a parabola with a focus at (0,5) and a directrix at x = -7 54. Suppose the population of a country is currently 8,100,000. Studies show thiscountry's population is increasing 2% each year.a. What exponential function would be a good model for this country'spopulation?b. Using the equation you found in part (a), how many years will it take for thecountry's population to reach 9 million? Round your answer to the nearesthundredth.