- What information do you obtain from the double digest XhoI/AlwNI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert in the correct orientation (how many bands, what are their sizes)? - What is your expectation if your plasmid has an insert in the incorrect orientation (how many bands, what are their sizes)?

Answers

Answer 1

Plasmids are used as vectors in recombination technology to transfer genes. Plasmids or the genes can be double digested by two restriction endonucleases simultaneously.

What are restriction endonucleases?

Restriction endonucleases are the enzymes that identify the precise DNA sequence to cleave them from the rest of the sequence.

If a plasmid is reclosed vector without an insert then only one band of size 2964 bp will be seen. A plasmid that has correct orientation will have two bands of sizes 1570 and 2100 bp.

If the plasmid has a sequence in incorrect orientation then will also result in two bands but of different sizes like 2740 bp and 940 bp.

Therefore, plasmids can be used as vectors.

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Related Questions

If non-series-rated overcurrent devices (standard molded-case circuit breakers) are to be installed in the main switchboard, breakers with adequate ____ must be installed in the panelboards.

Answers

Breakers with adequate short-circuit must be installed in the panelboards.

What are these equipment about?

Common Overcurrent Protection Devices (OCPDs) are known to be Breakers or fuses that are often used  to protect the full unit from too much current.

Note that Equipment such as switchboards and panelboards are often installed in an electrical system and they have to be of adequate measure of short-circuit current condition.

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13,Which hardware device is used in decoding the machine language version of an instruction in the Instruction Decode stage of the Fetch Execution Cycle

Answers

It should be the control unit

Which button on a desktop computer begins a reboot of the computer without power being removed from the computer's components?

Answers

Answer:

1. Click the Start button in the lower-left corner of your screen. You can also press the Windows key on your keyboard to open the Start menu.

2. Choose the Power icon.

3. When you click the power button, you will have the option to put your computer to sleep, restart it, or power it down.

Explanation:

Have a good day

A programmer used Scratch to create a program with three frog sprites. If a
user clicks any of the frogs, they make a "boing" sound and glide to a random
position in the stage panel.
In this scenario, what are two examples of an algorithm?
A. The combination of panels that compose the Scratch interface
B. The code that instructs the computer to move the sprite after it is
clicked
C. The user's decision about which frog to click
D. The code that generates a random location each time.

Answers

Answer:

D. The code that generates a random location each time.

Explanation:

An algorithm is a process or set of rules to be followed in calculations or other problem-solving operations, especially by a computer.

An algorithm is needed to get a random location.

The code that instructs the computer to move the sprite after it is clicked and the code that generates a random location each time are examples of an algorithm in this scenario. The correct options are B and D.

What is algorithm?

A set of instructions or rules that must be followed in order to complete a task or solve a problem is referred to as an algorithm.

The programmer in this scenario used Scratch to create a programme with three frog sprites that respond to user input by making a "boing" sound.

In this scenario, the combination of panels that comprise the Scratch interface and the user's decision about which frog to click are not examples of algorithms.

The panels that make up the Scratch interface are part of the programming environment, but they have no effect on the program's behaviour.

Similarly, the user's choice of which frog to click is a user input, but it does not form part of the algorithm that governs the program's behaviour.

Thus, the correct options are B and D.

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What is output by the following code?

for x in range (4):
for y in range (3):
print("*", end=" ")
print("")
Group of answer choices

* * * *
* * * *
* * * *
* * * *
* * * *

* * *
* * *
* * *
* * *

* * * * *
* * * * *
* * * * *
* * * * *

* * * *
* * * *
* * * *

Answers

Answer:

* * *

* * *

* * *

* * *

Explanation:

The inner loop will print 3 times *

For every outer loop, that 3 times * will be printed again => 4 times in this case

The end=" " is to add new line in each inner loop

The questions of economics address which of the following? Choose three answers.
who
what
where
when
why
how

Answers

Answer:

Economists address these three questions: (1) What goods and services should be produced to meet consumer needs? (2) How should they be produced, and who should produce them? (3) Who should receive goods and services? The answers to these questions depend on a country's economic system.

So the answer would be WHAT, WHY & WHO. Hope this helps

Explanation:

Program _______ graphically present the detailed sequence of steps needed to solve a programming problem.

Answers

Program Pseudocode graphically present the detailed sequence of steps needed to solve a programming problem.

What are Pseudocode?

These are known to be a set of codes that a program has graphically depicted all of its sequence of steps that can be sued to handle a programming problem.

Note that Pseudocode is a form of artificial and man made informal language that is often used by programmers to create  algorithms.

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Besides the Game Engine, name and describe three other game development tools. Then, briefly compare and contrast these tools.

Answers

The three other game development tools are:

Level editing tools.Scripting tools.Modeling tools.

What are Game development tools?

A game development tool is known to bee any kind of specialized application that has been made to help assists or boast the creation of a game.

Conclusively, Note that those free game creating software written above can a person to start making  their dream game among others.

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Jeremy knows that there is a keyboard shortcut in Word, but he does not know what it is. What should Jeremy do to find out what the keyboard shortcut is?

Answers

I think he should ask someone who uses word to help him ;)

You have double-clicked a device in Device Manager to open its Properties window. Where can you find information about IRQs the device might use

Answers

To find information about IRQs the device might use, make alteration to the View menu option and then change it to Resources by type.

How do one find information about IRQs?

The best way to see IRQ information in Windows is through the use of Device Manager.

Note that for one to be able to find information about IRQs the device might use, one has to makes some changes to the View menu option and then one can change it to Resources by type to be able to see the Interrupt request (IRQ) part.

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As a student you would most likely use the ________ collaboration tool set because it is free and takes not time to learn. Group of answer choices maximum

Answers

Collaboration toolsets are the programs and apps that make the work more effective and creative. Students should use a minimal collaboration toolset.

What is a minimal collaboration toolset?

A minimal collaboration toolset is a platform, application, or program that is free of cost and requires no time to learn. It is used in emails and other text platforms.

It shares content through email and shares document files. This minimal collaboration toolset uses all text but lacks voices and videos.

Therefore, the minimal collaboration toolset is the correct blank.

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Which term best describes the set that makes up an algorithm?
A. Steps
B. Documents
C. Statements
D. Patterns

Answers

Answer:

A

Explanation:

An algorithm is a series of steps to get to a final result.

The term that best describes the set that makes up an algorithm is steps. The correct option is A.

What is algorithm?

An algorithm is a set of step-by-step instructions that are followed in order to complete a specific task or solve a particular problem.

Each step in the algorithm is typically a distinct command or operation designed to accomplish a specific goal or modify a specific aspect of the data being processed.

Documents, statements, and patterns are not the best terms to describe the collection of elements that comprise an algorithm.

While an algorithm can be documented or described in written or visual form, the term "documents" does not adequately describe the nature of the instructions themselves.

Similarly, while an algorithm may be made up of individual commands or operations, the term "statements" does not convey the concept of a step-by-step process.

Finally, while an algorithm may contain repeating patterns or structures, the term "patterns" does not imply a linear sequence of steps that must be performed in a specific order.

Thus, the correct option is A.

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Another word for asking a question of your data is to _____ it.

Answers

Answer:

query

Explanation:

Your friend Cameron’s little sister is visually impaired. Cameron is worried that his sister will not be able to use technology and will be at a disadvantage in life. What could you tell Cameron to reassure him that his sister will still be able to use technology?

Answers

All modern technology has things to help disabled people

which of the following is a benifit of googling yourself ?

Answers

Answer:

you are protecting yourself from identity theft

You are writing an algorithm and want to tell the computer what to do if the
user does not perform an action. Which type of statement should you include
in your algorithm?
A. Return
B. When-if
C. If-else
D. And-or

Answers

Answer:

you are writing an algorithm and went to tell computer what to do If the return

The type of statement, i.e., if-else should be include in your algorithm. The correct option is C.

What is if-else statement?

An "if-else" statement is a conditional statement that determines whether a particular condition is true or false and then executes different code based on the outcome of that evaluation.

An "if-else" statement could be used in the context of a user action to check whether the user has performed a specific action and then execute different code depending on whether the user has performed that action or not.

To exit a function and return a value to the calling code, use a "return" statement. A "when-if" statement and a "and-or" statement are not standard programming constructs and are rarely used in code.

Thus, the correct option is C.

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The Director of your company is traveling out of the country with the company laptop. Which solution can assist with recovery in the event the laptop is stolen

Answers

What solutions does the question give for you to choose from?
I’d go for backup

One way to protect against a security threat to a computer system is to __________. Avoid external links with inconsistent URLs create a disaster recovery plan open online pop-up notifications post personal information online

Answers

One way to protect against a security threat to a computer system is to Avoid external links with inconsistent URLs.

What is malware?

Malware is any programme that is purposely designed to disrupt a computer, server, client, or computer network, leak private information, obtain unauthorised access to information or systems, deny users access to information, or otherwise interfere with the user's computer security and privacy.

One way to protect against a security threat to a computer system is to Avoid external links with inconsistent URLs. The reason for this is that such links may contain malware or spyware.

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What is the primary difference between sort and filter?

Answers

Answer:

Filter only shows the messages that match a particular criterion

The _____ standard uses multiple input/multiple output (MIMO) technology to boost performance.

Answers

802.11n standard uses multiple input/ MIMO technology.output (MIMO) technology to boost performance. Increases the speed of wireless local area network communications, as well as their dependability and range.

What is MIMO technology?

Multiple-Input MIMO Many-Input, Multiple-Output is a wireless technology that uses multiple transmitters and receivers to carry more data at once. MIMO is supported by all 802.11n wireless equipment.

The technology enables 802.11n to achieve faster rates than goods that do not have 802.11n.

Thus, 802.11n standard used for boosting performance in MIMO technology.

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20 Points!
Select the correct answer.
Look at this web page from the National Oceanic and Atmospheric Administration’s (NOAA) website. How does the page use contrast?

a web page on the NOAA website

A.
by providing a search box for users
B.
by using a consistent type of font for all text
C.
by using an image of an actual school of fish
D.
by using lighter font against a darker background

Answers

B. By using a consistent type of font for all text. Hope this helps and have a great day

Answer:

By using lighter font against a darker background

Explanation: Contrast, the state of being strikingly different from something else in juxtaposition or close association! Or opposites for short!

___ is a type of social engineering where a hacker lures individuals into entering personal information into a website controlled by the hacker.

Answers

A type of social engineering where a hacker lures unsuspecting individuals to enter personal information into a website controlled by the hacker is phishing.

What is phishing?

Phishing can be defined as a type of social engineering (cyber attack) which involves making an attempt to obtain sensitive user informations, especially by disguising one's self as a trustworthy entity in an electronic communication, which is usually over the Internet.

This ultimately implies that, a type of social engineering where a hacker lures unsuspecting individuals to enter personal information such as usernames, passwords and credit card details or bank account details into a website controlled by the hacker is called phishing.

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A program has a class Potato , which is derived from the class Vegetable , which is derived from the class Food . This is an example of _______

Answers

Inheritance

If you have a potato, it has the features/characteristics of a vegetable
A potato is healthy, and it gets this from being a vegetable
A vegetable gets its features/characteristics from food
A vegetable can be eaten, just like food
We can then say that a vegetable inherits the ability to be eaten from food
And that the potato inherits this from being a vegetable
The potato class inherits from the vegetable class the healthiness, but the vegetable class doesn’t inherit healthiness from the food class

Guys please tell me which cable is this?

Answers

Answer:

That might just be an direct internet cable

Explanation:

Answer:

it looks like a baseball bat to me

Explanation:

because if you swing it at someones forehead they will go to the disney park

Customer records containing names, addresses, phone numbers, and purchase histories would be most likely stored in: customer relational networks database management systems spreadsheets information processing networks transactional networks

Answers

Customer records are the personal information of the customer that is registered in a store. Customers' records in stores are stored in the database management systems.

What is a database management system?

A database management system (DBMS) is a computer-based system that stores and manages the data stored by the users. It uses software to save, retrieve and run data-related queries.

DBMS is used to store and manage a large amount of information and allows users to manipulate them. This allows the users to interact and access the data of the customers.

Therefore, the customer's records are stored in database management systems.

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1. The first Pong arcade game in the ______ is considered to be the start of modern video game industry

a) early 60s

b) early 70s

c) early 80s

d) early 90s

Answers

Answer:

B

Explanation:

The first pong game came out November 29, 1972

A machine learning cloud application provides meaningful data with the company's local customer information and purchase history. The company considers this data sensitive. How should the company manage this data when interfacing with the cloud service

Answers

Cloud storage is a service prototype that stores the data on remote storage sources generally on the net. The company can manage the data by keeping data on the local storage.

What is local storage and cloud storage?

Local storage is the storage place present in the system either in the hard drives or in the form of a solid-state drive (SSD). Cloud storage is the web storage platform that stores the data on the internet.

The cloud storage maintains, backs ups, and manages the files and folders stored on the cloud platform. Cloud storage is the virtual platform that is available to users based on the space needed.

Therefore, the data on local storage is managed when interfacing with the cloud system.

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A(n) _____ is an organization's management support system that combines many databases to improve management decision making. data mine information center data station data warehouse information network

Answers

A data warehouse is an organization's management support system that combines many databases to improve management decision making.

What is a management support system?

A decision support system, executive information system, as well as the knowledge-based or expert system functional capabilities are known to be factors that are all integrated into an  management support system.

Therefore saying that A data warehouse is an organization's management support system that combines many databases to improve management decision making is a true statement.

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Which subscription service complements App-ID by enabling you to configure the next-generation firewall to identify and control access to websites and to protect your organization from websites hosting malware and phishing pages.

Answers

The subscription service that complements App-ID by enabling you to configure the firewall to identify and control access to websites is  URL Filtering.

What is URL filtering?

The term URL filtering is known to function by looking at the similarities of all web traffic against a given URL filters.

Note that it is one that is made up of database of sites that users do access and as such, the subscription service that complements App-ID by enabling you to configure the firewall to identify and control access to websites is  URL Filtering.

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In the following citation for a website, which detail is optional, according to
MLA style?
Manufacturers Association for Plastics Processors. Home page. Web. 6 Mar.
2014 .
A. Web.
B. 6 Mar. 2014.
C.
D. Home page

Answers

According to MLA style, the detail which is optional in the given citation for a website is: C. mapping link.

What is an information source?

An information source can be defined as a system or medium through which information, knowledge and ideas may be gotten (generated) and used by an individual.

What are citations?

Citations can be defined as references or links to all of the sources from which an author, writer, or researcher got his or her information from.

The characteristics of a MLA citation.

Generally, MLA citation often comprises the following information:

Author's last name and first name.Title of source.Title of container.Other contributors.VersionThe name of the publisher.Publication date.Location.

According to MLA style, the detail which is optional in the given citation for a website is mapping link.

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Complete Question:

In the following citation for a website, which detail is optional, according to

MLA style?

Manufacturers Association for Plastics Processors. Home page. Web. 6 Mar.

2014 .

A. Web.

B. 6 Mar. 2014.

C. Mapping link

D. Home page

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