You are attempting to carry out a transposon mutagenesis experiment using the pRL27 system. The Donor strain you use was giving to you by a lab mate who has been culturing it continuously for several months. When you set up your control plates, you find that the Donor strain grows poorly on Luria DAP Agar. Your transformation efficiency is very poor for this attempt at mutageneis. What likely happened to the Donor strain

Answers

Answer 1

The most likely happened to the donor strain is that the transposon is active in the donor resulting in the gradual accumulation of mutations. The accumulation of mutations is promoted by the insertion of the transposon.

A donor strain is a bacterial strain that contains a mobilizable fragment of DNA called plasmid.

These strains (donor strains) exploit the transference properties of a conjugative plasmid to modify a particular recipient strain.

Transposons, also known as jumping genes, are mobile genetic elements that alter the genome of an organism by jumping and thus inserting them into genetic sequences (i.e., into protein-coding sequences).

In this case, the presence of a transposon in the donor strain affected the expression of many genes required for the donor strain in order to grow into Luria DAP Agar.

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Related Questions

Helpppppppp! Its biology related <3 help help !

Answers

C, this answer mentions the many benefits of the marine life and reefs in the ecosystem, therefore the only answer could be C because it speaks of preserving these organisms

a. both
b. neither
c. the inside of the cell
d. the beaker​

Answers

Answer:

c

Explanation:

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5) Which of these best describe a physical change? A) Waste products are formed. B) Biting into a piece of bread. Simple sugars in the small intestine are broken down into glucose. D) Saliva in the mouth breaks the bread into smaller molecules like maltose.​

Answers

Answer: Biting into a piece of bread

Explanation:

Answer:

B Biting into a peace of bread

Explanation:

I took the test and as well. the first physical thing that changes whenever any type of food goes into your mouth is the way it breaks apart/turns into a smaller part of the food so that your esaphgous can swallow it. Hope this helped :)

the division of genetic material in a eukaryotic cell is called

Answers

Answer:

Mitosis

Explanation:

Which items are TRUE about minerals? Select the three correct answers. (2 points)
Observing one property is often the best way to identify minerals,
Rocks are the components that make up minerals.
Minerals can only be identified by their color and hardness.
Minerals are the components that make up rocks.
O Minerals are naturally occurring substances
Minerals are made of one element or one compound of elements

Answers

Answer:

The correct answers are 1) minerals are the components that make up rocks. 2) Minerals can only be identified that make up minerals. 3) Minerals are made of one element or one compound of elements.

Explanation:

I took the test. I got this question wrong but after submitting it, the lesson should me the correct answer.

The items that are true about minerals are:

Minerals are naturally occurring substances.

Minerals are inorganic and homogeneous substances, which can be found on the surface of the Earth, formed in it naturally / spontaneously.

Minerals are made of one element or one compound of elements.

Minerals are found in various forms in nature since they can be of a single element, such as gold, silver, copper, or a combination of elements, such is the case of some chemical compounds such as quartz, which is formed by silicon and oxygen.

Minerals are the components that make up rocks.

A rock is a material that can be made up of a mineral or a combination of them, but its chemical composition is variable.

Therefore, we can conclude that a mineral is a substance produced by processes of inorganic nature which are made of one element or one compound of elements.

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If you throw a ball straight up into the air, when is the kinetic energy the greatest?

Question 4 options:

Right before you catch it after it falls back down.


When the ball reaches the highest point and stops briefly.


Half the distance on the path traveling back down.


Half the distance on the path traveling up in the air.

Answers

Half distence on the path traveling up in the air.

Explanation:

When the ball is on thr ground there and not moving there is no potential energy or kinetic energy, when the ball is tossed into the air it will reach maximum height which is determined by how much kinetic energy it has (air resistence is important however) when the ball reaches its velocitu is 0 and all of the energy in the system is potential energy, as the ball falls to the ground that potential energy is converted to kinetic energy

If a ball is thrown straight up into the air, the kinetic energy is greatest when the distance is half on the path travelling up in the air. Thus, the correct option is D.

What is Kinetic energy?

Kinetic energy is the energy associated with the body which is under motion. Kinetic energy is directly proportional to the distance, mass and velocity of the object.

When the ball is thrown on the ground and is not moving then there will be no potential energy or kinetic energy as the height and velocity is zero when the object is on ground. When the ball is tossed into the air, it will reach maximum height which is determined by the kinetic energy it has and the air resistance when the ball reaches zero velocity and the greatest kinetic energy is observed when the body attains half of the total distance on the path.

Therefore, the correct option is D.

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Which of the following is TRUE about ovarian cysts?

Answers

Answer:

d. All of the above

Explanation: is correct.

Answer:

ALL OF THE ABOVE

Explanation:

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Which two activities are practical applications of magnetism?
A. Separating steel nails from aluminum nails
B. Using the North Star to find which way is south
C. Hammering steel nails into wooden boards
D. Finding which way is north with a compass

Answers

Answer:

It is d and a

Explanation:

when nonsister chromatids exchange genes it is called:

Answers

Answer:Chromosomal crossover, or crossing over

Explanation:

Chromosomal crossover, or crossing over, is the exchange of genetic material during sexual reproduction between two homologous chromosomes' non-sister chromatids that results in recombinant chromosomes.

What might scientists learn about the evolution of human behavior from studying cognitive behavior in animals?

Answers

Answer:

When we gain insight into animal behavior, we are in a stronger position to understand vexing conservation problems, such as how to save endangered species, assess environmental quality, design nature preserves, and evaluate the importance of human-related threats to survival in otherwise fit animals.

Explanation:

Plant and animal cells have a lot in common. Compare and contrast the two cells. Select the features that you find in BOTH types of cells. Question 17 options: membrane, nucleus, cytoplasm, membrane, chloroplasts, nucleus cell wall, cell membrane, nucleus cell membrane and cell wall, chloroplasts.

Answers

Answer and explanation:

Animal cells do not have cell walls or chloroplasts but both plant and animal cells contains nucleus and cell membrane.

During the development of skeletal muscle cells, some myoblasts do not fuse and instead become ______ cells.

Answers

Answer:

It would become satellite

Differentiate How
does telophase II differ from
telophase I?

Answers

Answer: Telophase I is the ending phase of the first division of meiosis which would split the parent cell into two daughter cells while telophase II is the ending phase of the second division of meiosis which would split the two daughter cell into four daughter cells and resulting in the end of the process.

what part of the autonomic nervous system is represented in the given image?

Answers

There is no image attached to this question

1 point
10) Energy being stored in an object that has the potential to do work is
called
a. hydro energy
b. kinetic energy
c. thermal energy
d. potential energy

Answers

Answer: D. Potential Energy

Explanation:

The energy stored in that object has the potential of doing something/becoming kinetic energy.

PLS HURRY


Why are fossils important to developing a theory of evolution?

Answers

Answer:

Fossils are important evidence for evolution because they show that life on earth was once different from life found on earth today. ... Paleontologists can determine the age of fossils using methods like radiometric dating and categorize them to determine the evolutionary relationships between organisms

Explanation:

Fossils are important evidence for evolution because they show that life on earth was once different from life found on earth today. ... Paleontologists can determine the age of fossils using methods like radiometric dating and categorize them to determine the evolutionary relationships between organisms

The preserved remnants or traces of extinct animals, plants, and other organisms are known as fossils. Because they demonstrate that earlier life on Earth was distinct from the life observed today, fossils are crucial pieces of evidence for evolution.

What is evolution?

According to the idea of evolution, modern life forms have evolved from more ancient ones.

The original theory suggested that this occurred slowly and gradually, but fossil evidence suggests that the process occasionally went wrong and engaged in some very bizarre experiments.

Therefore, they demonstrate that earlier life on Earth was distinct from the life observed today, fossils are crucial pieces of evidence for evolution.

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Maxine miles runs a marathon in 2 hours and 40 minutes. most of the atp for this activity is supplied by:

Answers

Maxine miles runs a marathon in 2 hours and 40 minutes. Most of the ATP for this activity is supplied by AEROBIC glucose breakdown. ATP is the energy coin of the cell.

Cellular respiration refers to the metabolic processes by which aerobic cells produce ATP by using the energy stored in the chemical bonds of foods (e.g., glucose) and oxygen.

Muscle cells can produce ATP by both cellular respiration (aerobic conditions) and acid lactic fermentation (anaerobic conditions).

Acid lactic fermentation is a less efficient pathway than cellular respiration, but cellular respiration is slower than fermentation, thereby muscle cells use this pathway (acid lactic fermentation) when there is not enough oxygen in the body.

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what is the texture of a worker bee leg.​

Answers

The texture of a bee leg is like a shiny gloss to it with tiny fine hairs all over it so that pollen will stick.

what happened to the molecules when you moved the slider to 0°c ?

Answers

Answer:

: Liquid water molecules move more and more slowly until they begin to stick together to form an ice crystal.

Explanation:

Oi, Its worth 40 points, plus brainliest to best answer.


Use the graph and the information provided to answer the questions.
Stickleback fish protect themselves from predators with a thick covering of brownish plates. They feed on
small aquatic organisms and are preyed upon by bigger fish like trout. In a certain lake, the number of
stickleback fish was documented over a period of 30 years. During this time, the clarity of the lake water
also changed.



1. How did the occurrences of the different traits change over the 30-year period? Use evidence from
the graph to support your answer. Using what you know about natural selection and adaptation, what
generalization can you make based on these changes?

Answers

Explanation:

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just kidding the answer is

every time the differences will change because of the large fishes which kill the small ones and the fishes het big like in one big fish there will be 100 of small fishes like the megladon

during this blood also leaks and the colour of the lake changes

Answer:

In each instance, the variations in fish populations are subject to change due to the predation of larger fish on smaller ones. As a result, the surviving fish grow larger, akin to the manner in which the Megalodon can have hundreds of smaller fish within its body. This process can also result in the release of blood, which has the potential to alter the color of the surrounding water body.

Explanation:

Describe why enzymes are important in all living organisms?

Answers

Answer:

Enzymes are important because they make the chemical reactions in cells happen quickly.

Enzymes, by speeding up biological reactions, ensure that all necessary activities in a cell can take place simultaneously and prevent one particular activity from overtaking the entire cell or organism. This is possible because enzymes allow chemicals to react more specifically with only certain substances rather than reacting indiscriminately with any number of different substances within cells.

The most famous example of this enzyme effect is when stomach acid reacts with pepsin that turns food into a liquid. The body then uses this liquid for absorption through the intestines where nutrients are then used throughout the body. It's important for people to have enough enzymes in order to digest their food fully however if

Explanation:

Mutations in proto-oncogenes are generally _____ whereas mutations in tumor-suppressor alleles are generally _____.

Answers

Answer:

mutations in proto-oncogene are generally cancerous while mutation in tumor-suppressor alleles are recessive and result lost of function

which of these neurotransmitters does not bind to a plasma membrane receptor?

Answers

When neurotransmitter molecules bind to receptors in the plasma membrane of the receiving neurona. ion channels in the plasma membrane of the sending neuron open. b. ion channels in the plasma membrane of the receiving neuron open. c. the receiving neuron becomes more negative inside. d. the receiving neuron becomes more positive inside. e. vesicles in the synaptic terminal fuse to the plasma membrane of the sending neuron.

two other components of blood

Answers

It has four main components: plasma, red blood cells, white blood cells, and platelets. Blood has many different functions, including: transporting oxygen and nutrients to the lungs and tissues. forming blood clots to prevent excess blood loss.

the failure of sister chromatids to separate during meiosis is called:

Answers

Answer:

Nondisjunction in Meiosis: Nondisjunction occurs when homologous chromosomes or sister chromatids fail to separate during meiosis, resulting in an abnormal chromosome number.

Write an equation for the line with slope -5 and y-intercept of 14? O A. y = -5x - 14 O B. y = -5x + 14 HE 0 C. y = -5x + 18 O D. y = -5x + 6 HUSS​

Answers

Answer:

The equation of a line is written in the form y=mx+b. ”m” is the slope and “b” is the y-intercept. Plugging in the given values, the equation of the line is y=-5x + 14.

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Which of the following statements is TRUE?

If you have minor symptoms of PID, you do not need to be treated.
PID always causes severe pain.
*PID can result in having problems getting pregnant later in life.
PID occurs in males.

Answers

Answer:

c. PID can result in having problems getting pregnant later in life.

Explanation: is correct. post protected

During incubation, antimicrobials applied to the bacterial lawn become increasingly _________ as they diffuse out of the disk into the medium.

Answers

Answer:

Meee-boop

Explanation:

Ped0

By which time period did the average
temperature across Earth's surface rise
1.62 degrees F above the 20th century
average, the hottest period in the 136-
year average?
A. 1300s
B. 1500s
C. 1700s
D. 2000s

Answers

Answer:

C. 1700s

Explanation:

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Answer:

C. 1700s

Explanation:

How has the average temperature on Earth changed over time?

Earth's temperature has risen by 0.14° F (0.08° C) per decade since 1880, and the rate of warming over the past 40 years is more than twice that: 0.32° F (0.18° C) per decade since 1981. ... From 1900 to 1980 a new temperature record was set on average every 13.5 years; from 1981–2019, a new record was set every 3 years.

A piano player uses her hands for brief quick movements. Which muscle fiber type would you therefore predict to be predominant in these muscles

Answers

For brief quick movements, which muscle fiber type would you therefore predict to be predominant in these muscles...Fast twitch muscle fiber type.

There are three types of muscle tissue. 1. Cardiac 2. Smooth 3. skeletal muscles. The skeletal muscle are involved in voluntary movements of the body.

Since the player uses her hand in a voluntary way of movement, therefore she makes use of her skeletal muscles.

With regards to the skeletal muscles, there are two types with regards to speed, we have the slow twitch and the fast-twitch.

The fast-twitch muscle fiber aids in fast movement of the body but for shorter periods compared to the slow twitch.

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